| http://purl.uniprot.org/citations/18448537 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18448537 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18448537 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18448537 | http://www.w3.org/2000/01/rdf-schema#comment | "A myovirus-like temperate phage, PhiHAP-1, was induced with mitomycin C from a Halomonas aquamarina strain isolated from surface waters in the Gulf of Mexico. The induced cultures produced significantly more virus-like particles (VLPs) (3.73 x 10(10) VLP ml(-1)) than control cultures (3.83 x 10(7) VLP ml(-1)) when observed with epifluorescence microscopy. The induced phage was sequenced by using linker-amplified shotgun libraries and contained a genome 39,245 nucleotides in length with a G+C content of 59%. The PhiHAP-1 genome contained 46 putative open reading frames (ORFs), with 76% sharing significant similarity (E value of <10(-3)) at the protein level with other sequences in GenBank. Putative functional gene assignments included small and large terminase subunits, capsid and tail genes, an N6-DNA adenine methyltransferase, and lysogeny-related genes. Although no integrase was found, the PhiHAP-1 genome contained ORFs similar to protelomerase and parA genes found in linear plasmid-like phages with telomeric ends. Southern probing and PCR analysis of host genomic, plasmid, and PhiHAP-1 DNA indicated a lack of integration of the prophage with the host chromosome and a difference in genome arrangement between the prophage and virion forms. The linear plasmid prophage form of PhiHAP-1 begins with the protelomerase gene, presumably due to the activity of the protelomerase, while the induced phage particle has a circularly permuted genome that begins with the terminase genes. The PhiHAP-1 genome shares synteny and gene similarity with coliphage N15 and vibriophages VP882 and VHML, suggesting an evolutionary heritage from an N15-like linear plasmid prophage ancestor."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.00140-08"xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.00140-08"xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.00140-08"xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.org/dc/terms/identifier | "doi:10.1128/JVI.00140-08"xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/author | "Authement R.N."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/author | "Authement R.N."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/author | "Authement R.N."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/author | "Mobberley J.M."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/author | "Mobberley J.M."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/author | "Mobberley J.M."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/author | "Paul J.H."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/author | "Paul J.H."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/author | "Paul J.H."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/author | "Segall A.M."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/author | "Segall A.M."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/author | "Segall A.M."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/name | "J. Virol."xsd:string |
| http://purl.uniprot.org/citations/18448537 | http://purl.uniprot.org/core/name | "J. Virol."xsd:string |