http://purl.uniprot.org/citations/18454554 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/18454554 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/18454554 | http://www.w3.org/2000/01/rdf-schema#comment | "Mycobacterium tuberculosis ( Mtb) produces a number of sulfur-containing metabolites that contribute to its pathogenesis and ability to survive in the host. These metabolites are products of the sulfate assimilation pathway. CysQ, a 3'-phosphoadenosine-5'-phosphatase, is considered an important regulator of this pathway in plants, yeast, and other bacteria. By controlling the pools of 3'-phosphoadenosine 5'-phosphate (PAP) and 3'-phosphoadenosine 5'-phosphosulfate (PAPS), CysQ has the potential to modulate flux in the biosynthesis of essential sulfur-containing metabolites. Bioinformatic analysis of the Mtb genome suggests the presence of a CysQ homologue encoded by the gene Rv2131c. However, a recent biochemical study assigned the protein's function as a class IV fructose-1,6-bisphosphatase. In the present study, we expressed Rv2131c heterologously and found that the protein dephosphorylates PAP in a magnesium-dependent manner, with optimal activity observed between pH 8.5 and pH 9.5 using 0.5 mM MgCl 2. A sensitive electrospray ionization mass spectrometry-based assay was used to extract the kinetic parameters for PAP, revealing a K m (8.1 +/-3.1 microM) and k cat (5.4 +/-1.1 s (-1)) comparable to those reported for other CysQ enzymes. The second-order rate constant for PAP was determined to be over 3 orders of magnitude greater than those determined for myo-inositol 1-phosphate (IMP) and fructose 1,6-bisphosphate (FBP), previously considered to be the primary substrates of this enzyme. Moreover, the ability of the Rv2131c-encoded enzyme to dephosphorylate PAP and PAPS in vivo was confirmed by functional complementation of an Escherichia coli Delta cysQ mutant. Taken together, these studies indicate that Rv2131c encodes a CysQ enzyme that may play a role in mycobacterial sulfur metabolism."xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi702453s"xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi702453s"xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/author | "Hatzios S.K."xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/author | "Hatzios S.K."xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/author | "Iavarone A.T."xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/author | "Iavarone A.T."xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/author | "Bertozzi C.R."xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/author | "Bertozzi C.R."xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/pages | "5823-5831"xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/pages | "5823-5831"xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/title | "Rv2131c from Mycobacterium tuberculosis is a CysQ 3'-phosphoadenosine-5'-phosphatase."xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/title | "Rv2131c from Mycobacterium tuberculosis is a CysQ 3'-phosphoadenosine-5'-phosphatase."xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/volume | "47"xsd:string |
http://purl.uniprot.org/citations/18454554 | http://purl.uniprot.org/core/volume | "47"xsd:string |
http://purl.uniprot.org/citations/18454554 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18454554 |
http://purl.uniprot.org/citations/18454554 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18454554 |
http://purl.uniprot.org/citations/18454554 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18454554 |
http://purl.uniprot.org/citations/18454554 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18454554 |