| http://purl.uniprot.org/citations/1845897 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1845897 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1845897 | http://www.w3.org/2000/01/rdf-schema#comment | "In a previous study (J. Martinez, R. S. Lahijani, and S. C. St. Jeor, J. Virol. 63:233-241, 1989), we identified a late, unspliced 1.6-kb mRNA that maps to the HindIII R fragment of human cytomegalovirus (HCMV) AD169. In the present study, the direction of transcription of this mRNA was determined by Northern (RNA) analysis with strand-specific probes. Primer extension was used to precisely map the 5' end of the mRNA. An open reading frame (ORF) designated ORF 2-1, located 176 nucleotides downstream from the cap site of the 1.6-kb mRNA, was identified. A synthetic peptide was made representing a hydrophilic region in the amino terminus of ORF 2-1. Immunoprecipitation and Western immunoblot analysis of infected HEL cell lysates, using affinity-purified antibody to the peptide (anti-P2-1), detected a viral protein with an apparent molecular mass of 58 kDa late in infection. Further support for the presence of this protein in infected-cell lysates was obtained by an enzyme-linked immunosorbent assay. Expression of viral antigens in intact infected HEL cells was assessed by immunofluorescence. General cytoplasmic staining was observed at 62 h postinfection, in contrast to a localized staining observed in the nuclear and perinuclear region at 96 h postinfection."xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.65.1.373-381.1991"xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.65.1.373-381.1991"xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/author | "Adlish J.D."xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/author | "Adlish J.D."xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/author | "Lahijani R.S."xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/author | "Lahijani R.S."xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/author | "Otteson E.W."xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/author | "Otteson E.W."xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/author | "St Jeor S.C."xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/author | "St Jeor S.C."xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/name | "J. Virol."xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/name | "J. Virol."xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/pages | "373-381"xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/pages | "373-381"xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/title | "Characterization of a human cytomegalovirus 1.6-kilobase late mRNA and identification of its putative protein product."xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/title | "Characterization of a human cytomegalovirus 1.6-kilobase late mRNA and identification of its putative protein product."xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/volume | "65"xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://purl.uniprot.org/core/volume | "65"xsd:string |
| http://purl.uniprot.org/citations/1845897 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1845897 |
| http://purl.uniprot.org/citations/1845897 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1845897 |