| http://purl.uniprot.org/citations/18466202 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18466202 | http://www.w3.org/2000/01/rdf-schema#comment | "The pineal gland of zebrafish (Danio rerio) contains light-sensitive photoreceptor cells and plays an important role in the neuroendocrine system. The zebrafish exorhodopsin gene encodes a pineal-specific photoreceptive protein, whose promoter region harbors a cis-acting element, pineal expression-promoting element (PIPE), directing pineal-specific gene expression. For in vivo genetic studies on PIPE-binding proteins and their regulatory mechanisms, we generated a transgenic zebrafish line, Tg(P(20)-rh/P:gfp), that expresses green fluorescent protein (GFP) under the control of the zebrafish rhodopsin promoter fused with 20 PIPE repeats. In Tg(P(20)-rh/P:gfp) fish, PIPE-dependent gene expression is visualized by GFP fluorescence in the pineal gland along with PIPE-independent GFP signals in the retinal rod photoreceptors. The transgenic fish exhibit detectable and reproducible GFP fluorescence in the larval pineal gland by 5 days postfertilization. Antisense morpholino-mediated knock-down of a pineal transcription factor gene, otx5, suppresses pineal GFP expression in the transgenic line. In a pilot screen of N-ethyl-N-nitrosourea-treated fish of the GFP transgenic line, we isolated potential dominant mutations that cause attenuation of pineal GFP fluorescence with a marginal effect on the retinal GFP signal. The results suggest that the Tg(P(20)-rh/P:gfp) line will be useful for detecting deficits in PIPE-dependent gene expression in the pineal gland."xsd:string |
| http://purl.uniprot.org/citations/18466202 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1751-1097.2008.00363.x"xsd:string |
| http://purl.uniprot.org/citations/18466202 | http://purl.uniprot.org/core/author | "Fukada Y."xsd:string |
| http://purl.uniprot.org/citations/18466202 | http://purl.uniprot.org/core/author | "Kojima D."xsd:string |
| http://purl.uniprot.org/citations/18466202 | http://purl.uniprot.org/core/author | "Dowling J.E."xsd:string |
| http://purl.uniprot.org/citations/18466202 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18466202 | http://purl.uniprot.org/core/name | "Photochem Photobiol"xsd:string |
| http://purl.uniprot.org/citations/18466202 | http://purl.uniprot.org/core/pages | "1011-1015"xsd:string |
| http://purl.uniprot.org/citations/18466202 | http://purl.uniprot.org/core/title | "Probing pineal-specific gene expression with transgenic zebrafish."xsd:string |
| http://purl.uniprot.org/citations/18466202 | http://purl.uniprot.org/core/volume | "84"xsd:string |
| http://purl.uniprot.org/citations/18466202 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18466202 |
| http://purl.uniprot.org/citations/18466202 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18466202 |
| http://purl.uniprot.org/uniprot/#_Q6NYT9-mappedCitation-18466202 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18466202 |
| http://purl.uniprot.org/uniprot/Q6NYT9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18466202 |