| http://purl.uniprot.org/citations/18471090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18471090 | http://www.w3.org/2000/01/rdf-schema#comment | "Background informationAt fertilization in mammalian eggs, the sperm induces a series of Ca(2+) oscillations via the production of inositol 1,4,5-trisphosphate. Increased inositol 1,4,5-trisphosphate production appears to be triggered by a sperm-derived PLCzeta (phospholipase C-zeta) that enters the egg after gamete fusion. The specific phosphatidylinositol 4,5-bisphosphate hydrolytic activity of PLCzeta implies that DAG (diacylglycerol) production, and hence PKC (protein kinase C) stimulation, also occurs during mammalian egg fertilization. Fertilization-mediated increase in PKC activity has been demonstrated; however, its precise role is unclear.ResultsWe investigated PLCzeta- and fertilization-mediated generation of DAG in mouse eggs by monitoring plasma-membrane translocation of a fluorescent DAG-specific reporter. Consistent plasma-membrane DAG formation at fertilization, or after injection of physiological concentrations of PLCzeta, was barely detectable. However, when PLCzeta is overexpressed in eggs, significant plasma-membrane DAG production occurs in concert with a series of unexpected secondary high-frequency Ca(2+) oscillations. We show that these secondary Ca(2+) oscillations can be mimicked in a variety of situations by the stimulation of PKC and that they can be prevented by PKC inhibition. The way PKC leads to secondary Ca(2+) oscillations appears to involve Ca(2+) influx and the loading of thapsigargin-sensitive Ca(2+) stores.ConclusionsOur results suggest that overproduction of DAG in PLCzeta-injected eggs can lead to PKC-mediated Ca(2+) influx and subsequent overloading of Ca(2+) stores. These results suggest that DAG generation in the plasma membrane of fertilizing mouse eggs is minimized since it can perturb egg Ca(2+) homoeostasis via excessive Ca(2+) influx."xsd:string |
| http://purl.uniprot.org/citations/18471090 | http://purl.org/dc/terms/identifier | "doi:10.1042/bc20080033"xsd:string |
| http://purl.uniprot.org/citations/18471090 | http://purl.uniprot.org/core/author | "Yu Y."xsd:string |
| http://purl.uniprot.org/citations/18471090 | http://purl.uniprot.org/core/author | "Lai F.A."xsd:string |
| http://purl.uniprot.org/citations/18471090 | http://purl.uniprot.org/core/author | "Swann K."xsd:string |
| http://purl.uniprot.org/citations/18471090 | http://purl.uniprot.org/core/author | "Halet G."xsd:string |
| http://purl.uniprot.org/citations/18471090 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18471090 | http://purl.uniprot.org/core/name | "Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/18471090 | http://purl.uniprot.org/core/pages | "633-643"xsd:string |
| http://purl.uniprot.org/citations/18471090 | http://purl.uniprot.org/core/title | "Regulation of diacylglycerol production and protein kinase C stimulation during sperm- and PLCzeta-mediated mouse egg activation."xsd:string |
| http://purl.uniprot.org/citations/18471090 | http://purl.uniprot.org/core/volume | "100"xsd:string |
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