http://purl.uniprot.org/citations/18471277 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/18471277 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/18471277 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundDss1 (or Rpn15) is a recently identified subunit of the 26S proteasome regulatory particle. In addition to its function in the protein degradation machinery, it has been linked to BRCA2 (breast cancer susceptibility gene 2 product) and homologous DNA recombination, mRNA export, and exocytosis. While the fungal orthologues of Dss1 are not essential for viability, the significance of Dss1 in metazoans has remained unknown due to a lack of knockout animal models.ResultsIn the current study deletion of dss-1 was studied in Caenorhabditis elegans with a dss-1 loss-of-function mutant and dss-1 directed RNAi. The analysis revealed an essential role for dss-1 in oogenesis. In addition, dss-1 RNAi caused embryonic lethality and larval arrest, presumably due to loss of the dss-1 mRNA maternal contribution. DSS-1::GFP fusion protein localised primarily in the nucleus. No apparent effect on proteasome function was found in dss-1 RNAi treated worms. However, expression of the C. elegans dss-1 in yeast cells deleted for its orthologue SEM1 rescued their temperature-sensitive growth phenotype, and partially rescued the accumulation of polyubiquitinated proteins in these cells.ConclusionThe first knockout animal model for the gene encoding the proteasome subunit DSS-1/Rpn15/Sem1 is characterised in this study. In contrast to unicellular eukaryotes, the C. elegans dss-1 encodes an essential protein, which is required for embryogenesis, larval growth, and oogenesis, and which is functionally conserved with its yeast and human homologues."xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.org/dc/terms/identifier | "doi:10.1186/1471-213x-8-51"xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.org/dc/terms/identifier | "doi:10.1186/1471-213x-8-51"xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/author | "Holmberg C.I."xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/author | "Holmberg C.I."xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/author | "Jantti J."xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/author | "Jantti J."xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/author | "Palmen S."xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/author | "Palmen S."xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/author | "Pispa J."xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/author | "Pispa J."xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/name | "BMC Dev. Biol."xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/name | "BMC Dev. Biol."xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/pages | "51"xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/pages | "51"xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/title | "C. elegans dss-1 is functionally conserved and required for oogenesis and larval growth."xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/title | "C. elegans dss-1 is functionally conserved and required for oogenesis and larval growth."xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/volume | "8"xsd:string |
http://purl.uniprot.org/citations/18471277 | http://purl.uniprot.org/core/volume | "8"xsd:string |
http://purl.uniprot.org/citations/18471277 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18471277 |
http://purl.uniprot.org/citations/18471277 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18471277 |