| http://purl.uniprot.org/citations/18475022 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18475022 | http://www.w3.org/2000/01/rdf-schema#comment | "Valvular myofibroblasts (VMFs), being the most predominant cells in the cardiac valve, perform a variety of functions to maintain normal valvular physiology. These functions, such as contraction, proliferation, and wound repair, are all directly or indirectly mediated by intracellular Ca(2+) concentrations ([Ca (2+)](i)). Knowing how [Ca(2+)](i) is regulated by vasoactive agents in VMFs enriches the understanding of valvular biology in both health and diseases. In this study we examined the characteristics of purinergic agonist-induced [Ca(2+)] (i) responses and observed spontaneous Ca(2+) releases in cultured human VMFs. Secondary cultures of human mitral VMFs were incubated with the Ca(2+)-sensitive fluorescent indicator fura-2 or fluo-4 and visualized with fluorescence microscopy. Both ATP and UTP activated P(2Y2) receptors and induced endoplasmic reticulum (ER) Ca(2+) release and Ca(2+) influx. The lack of [Ca(2+)](i) responses in VMFs challenged with the selective P(2Y1) agonists ADPbetaS and 2-Me-S-ATP further supported that functional P(2Y2) receptors are responsible for the Ca(2+) signals. Finally, in a small number of VMFs spontaneous Ca(2+) releases in localized areas were observed. Blockade of the RyR elongated the latency period between each Ca(2+) releasing event, demonstrating the presence of functional RyRs in VMFs."xsd:string |
| http://purl.uniprot.org/citations/18475022 | http://purl.org/dc/terms/identifier | "doi:10.1536/ihj.49.221"xsd:string |
| http://purl.uniprot.org/citations/18475022 | http://purl.uniprot.org/core/author | "Wang X."xsd:string |
| http://purl.uniprot.org/citations/18475022 | http://purl.uniprot.org/core/author | "Liang W."xsd:string |
| http://purl.uniprot.org/citations/18475022 | http://purl.uniprot.org/core/author | "McDonald P."xsd:string |
| http://purl.uniprot.org/citations/18475022 | http://purl.uniprot.org/core/author | "McManus B."xsd:string |
| http://purl.uniprot.org/citations/18475022 | http://purl.uniprot.org/core/author | "van Breemen C."xsd:string |
| http://purl.uniprot.org/citations/18475022 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18475022 | http://purl.uniprot.org/core/name | "Int Heart J"xsd:string |
| http://purl.uniprot.org/citations/18475022 | http://purl.uniprot.org/core/pages | "221-236"xsd:string |
| http://purl.uniprot.org/citations/18475022 | http://purl.uniprot.org/core/title | "P2Y2 receptor-mediated Ca2+ signaling and spontaneous Ca2+ releases in human valvular myofibroblasts."xsd:string |
| http://purl.uniprot.org/citations/18475022 | http://purl.uniprot.org/core/volume | "49"xsd:string |
| http://purl.uniprot.org/citations/18475022 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18475022 |
| http://purl.uniprot.org/citations/18475022 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18475022 |
| http://purl.uniprot.org/uniprot/#_C6G7W1-mappedCitation-18475022 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18475022 |
| http://purl.uniprot.org/uniprot/#_C6G7W2-mappedCitation-18475022 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18475022 |
| http://purl.uniprot.org/uniprot/#_P41231-mappedCitation-18475022 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18475022 |
| http://purl.uniprot.org/uniprot/C6G7W2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18475022 |
| http://purl.uniprot.org/uniprot/P41231 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18475022 |
| http://purl.uniprot.org/uniprot/C6G7W1 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18475022 |