| http://purl.uniprot.org/citations/18483223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18483223 | http://www.w3.org/2000/01/rdf-schema#comment | "Nonsense-mediated mRNA decay (NMD) is a post-transcriptional surveillance process that eliminates mRNAs containing premature termination codons (PTCs). NMD has been hypothesized to impact on several aspects of cellular function; however, its importance in the context of a mammalian organism has not been addressed in detail. Here we use mouse genetics to demonstrate that hematopoietic-specific deletion of Upf2, a core NMD factor, led to the rapid, complete, and lasting cell-autonomous extinction of all hematopoietic stem and progenitor populations. In contrast, more differentiated cells were only mildly affected in Upf2-null mice, suggesting that NMD is mainly essential for proliferating cells. Furthermore, we show that UPF2 loss resulted in the accumulation of nonproductive rearrangement by-products from the Tcrb locus and that this, as opposed to the general loss of NMD, was particularly detrimental to developing T-cells. At the molecular level, gene expression analysis showed that Upf2 deletion led to a profound skewing toward up-regulated mRNAs, highly enriched in transcripts derived from processed pseudogenes, and that NMD impacts on regulated alternative splicing events. Collectively, our data demonstrate a unique requirement of NMD for organismal survival."xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.468808"xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/author | "Jacobsen S.E."xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/author | "Nielsen F.C."xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/author | "Weischenfeldt J."xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/author | "Nerlov C."xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/author | "Porse B.T."xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/author | "Damgaard I."xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/author | "Thoren L.A."xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/author | "Bryder D."xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/author | "Theilgaard-Monch K."xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/name | "Genes Dev"xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/pages | "1381-1396"xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/title | "NMD is essential for hematopoietic stem and progenitor cells and for eliminating by-products of programmed DNA rearrangements."xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://purl.uniprot.org/core/volume | "22"xsd:string |
| http://purl.uniprot.org/citations/18483223 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18483223 |
| http://purl.uniprot.org/citations/18483223 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18483223 |
| http://purl.uniprot.org/uniprot/#_A0A077S2U6-mappedCitation-18483223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18483223 |
| http://purl.uniprot.org/uniprot/#_A2AT37-mappedCitation-18483223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18483223 |
| http://purl.uniprot.org/uniprot/#_B2RRZ4-mappedCitation-18483223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18483223 |
| http://purl.uniprot.org/uniprot/#_D6RET5-mappedCitation-18483223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18483223 |
| http://purl.uniprot.org/uniprot/#_F6Q3G7-mappedCitation-18483223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18483223 |
| http://purl.uniprot.org/uniprot/#_Q3USS1-mappedCitation-18483223 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18483223 |