| http://purl.uniprot.org/citations/18501926 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18501926 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18501926 | http://www.w3.org/2000/01/rdf-schema#comment | "The Escherichia coli chromosomal relBE operon encodes a toxin-antitoxin system, which is autoregulated by its protein products, RelB and RelE. RelB acts as a transcriptional repressor and RelE functions as a cofactor to enhance the repressor activity of RelB. Here, we present the NMR-derived structure of a RelB dimer and show that a RelB dimer recognizes a hexad repeat in the palindromic operator region through a ribbon-helix-helix motif. Our biochemical data show that two weakly associated RelB dimers bind to the adjacent repeats in the 3'-site of the operator (O(R)) at a moderate affinity (K(d), approximately 10(-5) M). However, in the presence of RelE, a RelB tetramer binds two distinct binding sites within the operator region, each with an enhanced affinity (K(d), approximately 10(-6) M for the low-affinity site, O(L), and 10(-8) M for the high-affinity site, O(R)). We propose that the enhanced affinity for the operator element is mediated by a cooperative DNA binding by a pair of RelB dimers and that the interaction between RelB dimers is strongly augmented by the presence of the cognate toxin RelE."xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2008.04.039"xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2008.04.039"xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/author | "Inouye M."xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/author | "Inouye M."xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/author | "Li G.Y."xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/author | "Li G.Y."xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/author | "Ikura M."xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/author | "Ikura M."xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/pages | "107-119"xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/pages | "107-119"xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/title | "Structural mechanism of transcriptional autorepression of the Escherichia coli RelB/RelE antitoxin/toxin module."xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/title | "Structural mechanism of transcriptional autorepression of the Escherichia coli RelB/RelE antitoxin/toxin module."xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/volume | "380"xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://purl.uniprot.org/core/volume | "380"xsd:string |
| http://purl.uniprot.org/citations/18501926 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18501926 |
| http://purl.uniprot.org/citations/18501926 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18501926 |