| http://purl.uniprot.org/citations/18515495 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18515495 | http://www.w3.org/2000/01/rdf-schema#comment | "As successful generation of insulin-producing cells could be used for diabetes treatment, a concerted effort is being made to understand the molecular programs underlying islet beta-cell formation and function. The closely related MafA and MafB transcription factors are both key mammalian beta-cell regulators. MafA and MafB are co-expressed in insulin+beta-cells during embryogenesis, while in the adult pancreas only MafA is produced in beta-cells and MafB in glucagon+alpha-cells. MafB-/-animals are also deficient in insulin+ and glucagon+ cell production during embryogenesis. However, only MafA over-expression selectively induced endogenous Insulin mRNA production in cell line-based assays, while MafB specifically promoted Glucagon expression. Here, we analyzed whether these factors were sufficient to induce insulin+ and/or glucagon+ cell formation within embryonic endoderm using the chick in ovo electroporation assay. Ectopic expression of MafA, but not MafB, promoted Insulin production; however, neither MafA nor MafB were capable of inducing Glucagon. Co-electroporation of MafA with the Ngn3 transcription factor resulted in the development of more organized cell clusters containing both insulin- and glucagon-producing cells. Analysis of chimeric proteins of MafA and MafB demonstrated that chick Insulin activation depended on sequences within the MafA C-terminal DNA-binding domain. MafA was also bound to Insulin and Glucagon transcriptional control sequences in mouse embryonic pancreas and beta-cell lines. Collectively, these results demonstrate a unique ability for MafA to independently activate Insulin transcription."xsd:string |
| http://purl.uniprot.org/citations/18515495 | http://purl.org/dc/terms/identifier | "doi:10.1677/joe-08-0063"xsd:string |
| http://purl.uniprot.org/citations/18515495 | http://purl.uniprot.org/core/author | "Guo M."xsd:string |
| http://purl.uniprot.org/citations/18515495 | http://purl.uniprot.org/core/author | "Stein R."xsd:string |
| http://purl.uniprot.org/citations/18515495 | http://purl.uniprot.org/core/author | "Gu G."xsd:string |
| http://purl.uniprot.org/citations/18515495 | http://purl.uniprot.org/core/author | "Artner I."xsd:string |
| http://purl.uniprot.org/citations/18515495 | http://purl.uniprot.org/core/author | "Hang Y."xsd:string |
| http://purl.uniprot.org/citations/18515495 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18515495 | http://purl.uniprot.org/core/name | "J Endocrinol"xsd:string |
| http://purl.uniprot.org/citations/18515495 | http://purl.uniprot.org/core/pages | "271-279"xsd:string |
| http://purl.uniprot.org/citations/18515495 | http://purl.uniprot.org/core/title | "MafA is a dedicated activator of the insulin gene in vivo."xsd:string |
| http://purl.uniprot.org/citations/18515495 | http://purl.uniprot.org/core/volume | "198"xsd:string |
| http://purl.uniprot.org/citations/18515495 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18515495 |
| http://purl.uniprot.org/citations/18515495 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18515495 |
| http://purl.uniprot.org/uniprot/#_Q3V2C2-mappedCitation-18515495 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18515495 |
| http://purl.uniprot.org/uniprot/#_P54841-mappedCitation-18515495 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18515495 |
| http://purl.uniprot.org/uniprot/#_Q8CF90-mappedCitation-18515495 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18515495 |
| http://purl.uniprot.org/uniprot/P54841 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18515495 |
| http://purl.uniprot.org/uniprot/Q3V2C2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18515495 |
| http://purl.uniprot.org/uniprot/Q8CF90 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18515495 |