| http://purl.uniprot.org/citations/18558712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18558712 | http://www.w3.org/2000/01/rdf-schema#comment | "The highly conserved RecQ helicases are essential for the maintenance of genomic stability. Werner syndrome protein, WRN, is one of five human RecQ helicase homologues, and a deficiency of the protein causes a hereditary premature aging disorder that is characterized by genomic instability. A WRN orthologue, wrn-1 lacking the exonuclease domain, has been identified in the nematode Caenorhabditis elegans. wrn-1(RNAi) in C. elegans has a shortened life span, increased sensitivity to DNA damage, and accelerated aging phenotypes. However, little is known about its enzymatic activity. We purified the recombinant C. elegans WRN-1 protein (CeWRN-1) and then investigated its substrate specificity in vitro to improve our understanding of its function in vivo. We found that CeWRN-1 is an ATP-dependent 3'-5' helicase capable of unwinding a variety of DNA structures such as forked duplexes, Holliday junctions, bubble substrates, D-loops, and flap duplexes, and 3'-tailed duplex substrates. Distinctly, CeWRN-1 is able to unwind a long forked duplex compared to human WRN. Furthermore, CeWRN-1 helicase activity on a long DNA duplex is stimulated by C. elegans replication protein A (CeRPA) that is shown to interact with CeWRN-1 by a dot blot. The ability of CeWRN-1 to unwind these DNA structures may improve the access for DNA repair and replication proteins that are important for preventing the accumulation of abnormal structures, contributing to genomic stability."xsd:string |
| http://purl.uniprot.org/citations/18558712 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi800197m"xsd:string |
| http://purl.uniprot.org/citations/18558712 | http://purl.uniprot.org/core/author | "Bohr V.A."xsd:string |
| http://purl.uniprot.org/citations/18558712 | http://purl.uniprot.org/core/author | "Ahn B."xsd:string |
| http://purl.uniprot.org/citations/18558712 | http://purl.uniprot.org/core/author | "Hyun M."xsd:string |
| http://purl.uniprot.org/citations/18558712 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18558712 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/18558712 | http://purl.uniprot.org/core/pages | "7583-7593"xsd:string |
| http://purl.uniprot.org/citations/18558712 | http://purl.uniprot.org/core/title | "Biochemical characterization of the WRN-1 RecQ helicase of Caenorhabditis elegans."xsd:string |
| http://purl.uniprot.org/citations/18558712 | http://purl.uniprot.org/core/volume | "47"xsd:string |
| http://purl.uniprot.org/citations/18558712 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18558712 |
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| http://purl.uniprot.org/uniprot/Q19537#attribution-FC5D3E551073D6D29D54793D8B466B86 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/18558712 |
| http://purl.uniprot.org/uniprot/Q19546#attribution-31FF44F3DEE20567FAEDAF24B826EB29 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/18558712 |
| http://purl.uniprot.org/uniprot/Q95Y97#attribution-FC5D3E551073D6D29D54793D8B466B86 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/18558712 |
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| http://purl.uniprot.org/uniprot/#_Q19546-mappedCitation-18558712 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18558712 |
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| http://purl.uniprot.org/uniprot/Q95Y97 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18558712 |
| http://purl.uniprot.org/uniprot/Q19537 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18558712 |
| http://purl.uniprot.org/uniprot/Q19546 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18558712 |