| http://purl.uniprot.org/citations/18562585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18562585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18562585 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18562585 | http://www.w3.org/2000/01/rdf-schema#comment | "Sequence analysis of the endoproteolytic cleavage site within the hemagglutinin (HA) precursor protein HA(0) is fundamental for studies of the molecular biology of influenza A viruses, in particular, for molecular pathotyping of subtype H5 and H7 isolates. A current problem for routine diagnostics is the emergence of new strains of the H5 or H7 subtype or even other subtypes which escape detection by commonly used reverse transcription-PCR (RT-PCR) protocols. Here, the first pan-HA (PanHA) RT-PCR assay targeting the HA(0) cleavage site of influenza A viruses of all 16 HA subtypes is reported. The assay was assessed in comparison to H5 and H7 subtype-specific RT-PCRs for the HA(0) cleavage site and a real-time RT-PCR detecting the M gene. A panel of 92 influenza A viruses was used for validation. Sequence data for influenza A viruses from 32 allantoic fluid samples and 11 diagnostic swab samples of all 16 HA subtypes were generated by direct sequencing of the PanHA RT-PCR products. The results demonstrate that the new PanHA RT-PCR assay--followed by cycle sequencing--can complement existing methods and strengthen the reliability of influenza A virus diagnostics, allowing both molecular pathotyping (H5 and H7) and subtyping (non-H5 or -H7) within a single approach."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.org/dc/terms/identifier | "doi:10.1128/JCM.00466-08"xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.org/dc/terms/identifier | "doi:10.1128/jcm.00466-08"xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.org/dc/terms/identifier | "doi:10.1128/jcm.00466-08"xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Hoffmann B."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Hoffmann B."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Hoffmann B."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Beer M."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Beer M."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Beer M."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Grund C."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Grund C."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Grund C."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Gall A."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Gall A."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Gall A."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Harder T."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Harder T."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/author | "Harder T."xsd:string |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18562585 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |