| http://purl.uniprot.org/citations/18574469 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18574469 | http://www.w3.org/2000/01/rdf-schema#comment | "Sphingosine kinase 1 (SPHK1) is overexpressed in solid tumors and leukemia. However, the mechanism of SPHK1 overexpression by oncogenes has not been defined. We found that v-Src-transformed NIH3T3 cells showed a high SPHK1 mRNA, SPHK1 protein and SPHK enzyme activity. siRNA of SPHK1 inhibited the growth of v-Src-NIH3T3, suggesting the involvement of SPHK1 in v-Src-induced oncogenesis. v-Src-NIH3T3 showed activations of protein kinase C-alpha, signal transducers and activators of transcription 3 and c-Jun NH(2)-terminal kinase. Their inhibition suppressed SPHK1 expression in v-Src-NIH3T3, whereas their overexpression increased SPHK1 mRNA in NIH3T3. Unexpectedly, the nuclear run-on assay and the promoter analysis using 5'-promoter region of mouse SPHK1 did not show any significant difference between mock- and v-Src-NIH3T3. Furthermore, the half-life of SPHK1 mRNA in mock-NIH3T3 was nearly 15 min, whereas that of v-Src-NIH3T3 was much longer. Examination of two AU-rich region-binding proteins, AUF1 and HuR, that regulate mRNA decay reciprocally, showed decreased total AUF1 protein associated with increased tyrosine-phosphorylated form and increased serine-phosphorylated HuR protein in v-Src-NIH3T3. Modulation of AUF1 and HuR by their overexpression or siRNA revealed that SPHK1 mRNA in v-Src- and mock-NIH3T3 was regulated reciprocally by these factors. Our results showed, for the first time, a novel mechanism of v-Src-induced SPHK1 overexpression."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.org/dc/terms/identifier | "doi:10.1038/onc.2008.198"xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/author | "Ito H."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/author | "Gao S."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/author | "Murakami M."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/author | "Suzuki M."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/author | "Kimura A."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/author | "Nozawa Y."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/author | "Banno Y."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/author | "Kojima T."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/author | "Sobue S."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/author | "Furuhata A."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/author | "Murate T."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/author | "Takagi A."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/name | "Oncogene"xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/pages | "6023-6033"xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/title | "v-Src oncogene product increases sphingosine kinase 1 expression through mRNA stabilization: alteration of AU-rich element-binding proteins."xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://purl.uniprot.org/core/volume | "27"xsd:string |
| http://purl.uniprot.org/citations/18574469 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18574469 |
| http://purl.uniprot.org/citations/18574469 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18574469 |
| http://purl.uniprot.org/uniprot/#_P70372-mappedCitation-18574469 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18574469 |
| http://purl.uniprot.org/uniprot/#_G5E8G0-mappedCitation-18574469 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18574469 |
| http://purl.uniprot.org/uniprot/#_G3X9W0-mappedCitation-18574469 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18574469 |