Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/18586020http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18586020http://www.w3.org/2000/01/rdf-schema#comment"

Background

L-Dopa decarboxylase (DDC) is a pyridoxal 5'-phosphate-dependent enzyme that was found to be involved in many malignancies. The aim of this study was to investigate the mRNA expression levels of DDC in prostate tissues and to evaluate its clinical utility in prostate cancer (CaP).

Methods

Total RNA was isolated from 118 tissue specimens from benign prostate hyperplasia (BPH) and CaP patients and a highly sensitive quantitative real-time RT-PCR (qRT-PCR) method for DDC mRNA quantification has been developed using the SYBR Green chemistry. LNCaP prostate cancer cell line was used as a calibrator and GAPDH as a housekeeping gene.

Results

DDC was found to be overexpressed, at the mRNA level, in the specimens from prostate cancer patients, in comparison to those from benign prostate hyperplasia patients (p<0.001). Logistic regression and ROC analysis have demonstrated that the DDC expression has significant discriminatory value between CaP and BPH (p<0.001). DDC expression status was compared with other established prognostic factors, in prostate cancer. High expression levels of DDC were found more frequently in high Gleason's score tumors (p=0.022) as well as in advanced stage patients (p=0.032).

Conclusions

Our data reveal the potential of DDC expression, at the mRNA level, as a novel biomarker in prostate cancer."xsd:string
http://purl.uniprot.org/citations/18586020http://purl.org/dc/terms/identifier"doi:10.1016/j.clinbiochem.2008.04.026"xsd:string
http://purl.uniprot.org/citations/18586020http://purl.uniprot.org/core/author"Scorilas A."xsd:string
http://purl.uniprot.org/citations/18586020http://purl.uniprot.org/core/author"Fragoulis E.G."xsd:string
http://purl.uniprot.org/citations/18586020http://purl.uniprot.org/core/author"Avgeris M."xsd:string
http://purl.uniprot.org/citations/18586020http://purl.uniprot.org/core/author"Koutalellis G."xsd:string
http://purl.uniprot.org/citations/18586020http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18586020http://purl.uniprot.org/core/name"Clin Biochem"xsd:string
http://purl.uniprot.org/citations/18586020http://purl.uniprot.org/core/pages"1140-1149"xsd:string
http://purl.uniprot.org/citations/18586020http://purl.uniprot.org/core/title"Expression analysis and clinical utility of L-Dopa decarboxylase (DDC) in prostate cancer."xsd:string
http://purl.uniprot.org/citations/18586020http://purl.uniprot.org/core/volume"41"xsd:string
http://purl.uniprot.org/citations/18586020http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18586020
http://purl.uniprot.org/citations/18586020http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/18586020
http://purl.uniprot.org/uniprot/#_A0A087WV24-mappedCitation-18586020http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18586020
http://purl.uniprot.org/uniprot/#_A0A7L4XSC9-mappedCitation-18586020http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18586020
http://purl.uniprot.org/uniprot/#_A0A7L4XT01-mappedCitation-18586020http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18586020
http://purl.uniprot.org/uniprot/#_A0A7L4XT53-mappedCitation-18586020http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18586020
http://purl.uniprot.org/uniprot/#_A0A7L4XUZ5-mappedCitation-18586020http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18586020
http://purl.uniprot.org/uniprot/#_A0A7L4XVG2-mappedCitation-18586020http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18586020
http://purl.uniprot.org/uniprot/#_A0A7L4XVT7-mappedCitation-18586020http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18586020
http://purl.uniprot.org/uniprot/#_A0A0S2Z3N4-mappedCitation-18586020http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18586020
http://purl.uniprot.org/uniprot/#_A0A0S2Z3P0-mappedCitation-18586020http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18586020
http://purl.uniprot.org/uniprot/#_A0A7M2JGN3-mappedCitation-18586020http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18586020
http://purl.uniprot.org/uniprot/#_A0A3S5WLQ3-mappedCitation-18586020http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18586020
▶