http://purl.uniprot.org/citations/18588553 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/18588553 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/18588553 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundPan-allergens like profilins, calcium-binding proteins (CBPs), and nonspecific lipid transfer proteins have been suggested as possible specific markers for multiple pollen sensitizations, and could be used to predict cross-sensitization/poly-sensitization to several pollen allergens. Therefore, the purification and characterization of cross-reacting allergens in pollen is an extremely important task towards correct allergy diagnosis.MethodsNew pan-allergens were identified by screening a ragweed pollen cDNA library with sera of patients allergic to mugwort pollen. Resulting proteins were cloned, expressed, purified and characterized.ResultsWe report complete cDNA sequences of two profilin isoforms (Amb a 8.01 and Amb a 8.02), two isoforms of a 2EF-hand CBP (Amb a 9.01 and Amb a 9.02), a new 3EF-hand CBP (Amb a 10) from ragweed pollen and a 2EF-hand CBP from mugwort (Art v 5). All these proteins were expressed in Escherichia coli, purified to homogeneity and characterized by biochemical and immunological means.ConclusionsThe identified proteins are novel pan-allergens and can be used as diagnostic markers for polysensitization and used in component-resolved diagnosis."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1398-9995.2008.01635.x"xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1398-9995.2008.01635.x"xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Ferreira F."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Ferreira F."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Richter K."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Richter K."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Ebner C."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Ebner C."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Mari A."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Mari A."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Briza P."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Briza P."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Wopfner N."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Wopfner N."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Wallner M."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Wallner M."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Vogel L."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Vogel L."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Gruber P."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/author | "Gruber P."xsd:string |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
http://purl.uniprot.org/citations/18588553 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |