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http://purl.uniprot.org/citations/18602030http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18602030http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18602030http://www.w3.org/2000/01/rdf-schema#comment"We carried out a comparative proteomic study to explore the molecular mechanisms that underlie the defense response of Douglas-fir (DF, Pseudotsuga menziesii) to laminated root rot, a disease caused by Phellinus sulphurascens. 2-DE was conducted on proteins extracted from roots of laboratory-grown, young DF seedlings inoculated with P. sulphurascens. A total of 1303 proteins was detected in 7 dpi infected and uninfected root samples. Among these 1303 proteins, 277 showed differential expression that was statistically significant (p<0.05). Of these 277 proteins, 74 upregulated and 85 downregulated proteins showed at least a two-fold change from controls. Forty seven upregulated and 23 downregulated proteins were selected to be excised and analyzed using LC-MS/MS followed by peptide matching. Our results indicate that the major proteins differentially expressed in P. sulphurascens-infected DF seedlings include those in the following functional groups: disease/defense (27%), metabolism (16%), transcription factors (11%), signal transduction (10%), secondary metabolism (7%) and energy (4%). A number of additional proteins involved in cell structure (3%) and protein synthesis (3%) were also identified. By providing an initial database of candidate pathogenesis-related proteins for the DF-Phellinus sulphurascens pathosystem the results of this study will enable future detailed investigation of gene expression and function."xsd:string
http://purl.uniprot.org/citations/18602030http://purl.org/dc/terms/identifier"doi:10.1016/j.jprot.2008.06.004"xsd:string
http://purl.uniprot.org/citations/18602030http://purl.org/dc/terms/identifier"doi:10.1016/j.jprot.2008.06.004"xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/author"Ekramoddoullah A.K.M."xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/author"Ekramoddoullah A.K.M."xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/author"Islam M.A."xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/author"Islam M.A."xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/author"Sturrock R.N."xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/author"Sturrock R.N."xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/name"J. Proteomics"xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/name"J. Proteomics"xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/pages"425-438"xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/pages"425-438"xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/title"A proteomics approach to identify proteins differentially expressed in Douglas-fir seedlings infected by Phellinus sulphurascens."xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/title"A proteomics approach to identify proteins differentially expressed in Douglas-fir seedlings infected by Phellinus sulphurascens."xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/volume"71"xsd:string
http://purl.uniprot.org/citations/18602030http://purl.uniprot.org/core/volume"71"xsd:string
http://purl.uniprot.org/citations/18602030http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18602030
http://purl.uniprot.org/citations/18602030http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18602030
http://purl.uniprot.org/citations/18602030http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/18602030
http://purl.uniprot.org/citations/18602030http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/18602030