| http://purl.uniprot.org/citations/18603785 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18603785 | http://www.w3.org/2000/01/rdf-schema#comment | "Laminins are a family of large heterotrimeric glycoproteins comprising alpha, beta, and gamma chains. To determine the molecular mechanisms underlying chain assembly in vitro, we expressed human laminin-332 subunits in an insect cell-free translation system. We successfully produced the beta3-gamma2 heterodimer and the alpha3-beta3-gamma2 heterotrimer of the laminin coiled-coil (LCC) domain following co-translation of each chain. The alpha3-beta3 and the alpha3-gamma2 heterodimer were not detected, suggesting that the alpha3 chain can assemble with only beta3-gamma2 heterodimer to form a heterotrimer via disulfide bonds. These results are consistent with those of a previous report indicating that laminin chain assembly proceeds through the beta-gamma heterodimer to the alpha-beta-gamma heterotrimer in vivo. We suggest that the cell-free translation system is a valid system with which to study the mechanisms underlying laminin chain assembly."xsd:string |
| http://purl.uniprot.org/citations/18603785 | http://purl.org/dc/terms/identifier | "doi:10.1271/bbb.80108"xsd:string |
| http://purl.uniprot.org/citations/18603785 | http://purl.uniprot.org/core/author | "Ito M."xsd:string |
| http://purl.uniprot.org/citations/18603785 | http://purl.uniprot.org/core/author | "Kitagawa Y."xsd:string |
| http://purl.uniprot.org/citations/18603785 | http://purl.uniprot.org/core/author | "Kadowaki T."xsd:string |
| http://purl.uniprot.org/citations/18603785 | http://purl.uniprot.org/core/author | "Ezure T."xsd:string |
| http://purl.uniprot.org/citations/18603785 | http://purl.uniprot.org/core/author | "Niimi T."xsd:string |
| http://purl.uniprot.org/citations/18603785 | http://purl.uniprot.org/core/author | "Phan H.P."xsd:string |
| http://purl.uniprot.org/citations/18603785 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18603785 | http://purl.uniprot.org/core/name | "Biosci Biotechnol Biochem"xsd:string |
| http://purl.uniprot.org/citations/18603785 | http://purl.uniprot.org/core/pages | "1847-1852"xsd:string |
| http://purl.uniprot.org/citations/18603785 | http://purl.uniprot.org/core/title | "Expression and chain assembly of human laminin-332 in an insect cell-free translation system."xsd:string |
| http://purl.uniprot.org/citations/18603785 | http://purl.uniprot.org/core/volume | "72"xsd:string |
| http://purl.uniprot.org/citations/18603785 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18603785 |
| http://purl.uniprot.org/citations/18603785 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18603785 |
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| http://purl.uniprot.org/uniprot/#_B0YJ32-mappedCitation-18603785 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18603785 |
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