| http://purl.uniprot.org/citations/18615730 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18615730 | http://www.w3.org/2000/01/rdf-schema#comment | "SummaryCombining high-content screening (HCS) with random gene-trap mutagenesis could be a powerful tool to investigate transcriptional networks, cell signaling, chemical genetics, and developmental processes. However, a critical limitation has been poor quantification of reporter expression per cell. To overcome this hurdle, we generated a variety of Gtx-based expression cassettes and re-evaluated translational enhancement of arrayed Gtx segments in tandem by HCS. We then modified the cassette into a new polyA trap vector, which consists of a variant of yellow fluorescent protein, Venus, in combination with the Gtx segments. Expression of Venus was detected in about 60% of trapped genes assayed in embryonic stem cell (ESC) cultures, comparable to expression screening of LacZ-based vectors. Furthermore, tetraploid aggregations using a clone encoding a gene-trap insertion into Twist2 demonstrated identical spatiotemporal expression between Venus and Twist2. This highly sensitive reporter system is amenable to high-throughput expression-based real-time HCS including single cell analyses."xsd:string |
| http://purl.uniprot.org/citations/18615730 | http://purl.org/dc/terms/identifier | "doi:10.1002/dvg.20404"xsd:string |
| http://purl.uniprot.org/citations/18615730 | http://purl.uniprot.org/core/author | "Lan Q."xsd:string |
| http://purl.uniprot.org/citations/18615730 | http://purl.uniprot.org/core/author | "Stanford W.L."xsd:string |
| http://purl.uniprot.org/citations/18615730 | http://purl.uniprot.org/core/author | "Tanaka T.S."xsd:string |
| http://purl.uniprot.org/citations/18615730 | http://purl.uniprot.org/core/author | "Zandstra P.W."xsd:string |
| http://purl.uniprot.org/citations/18615730 | http://purl.uniprot.org/core/author | "Davey R.E."xsd:string |
| http://purl.uniprot.org/citations/18615730 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18615730 | http://purl.uniprot.org/core/name | "Genesis"xsd:string |
| http://purl.uniprot.org/citations/18615730 | http://purl.uniprot.org/core/pages | "347-356"xsd:string |
| http://purl.uniprot.org/citations/18615730 | http://purl.uniprot.org/core/title | "Development of a gene-trap vector with a highly sensitive fluorescent protein reporter system for expression profiling."xsd:string |
| http://purl.uniprot.org/citations/18615730 | http://purl.uniprot.org/core/volume | "46"xsd:string |
| http://purl.uniprot.org/citations/18615730 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18615730 |
| http://purl.uniprot.org/citations/18615730 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18615730 |
| http://purl.uniprot.org/uniprot/#_A5D6P6-mappedCitation-18615730 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18615730 |
| http://purl.uniprot.org/uniprot/#_Q9D030-mappedCitation-18615730 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18615730 |
| http://purl.uniprot.org/uniprot/Q9D030 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18615730 |
| http://purl.uniprot.org/uniprot/A5D6P6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18615730 |