| http://purl.uniprot.org/citations/18620009 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18620009 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18620009 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18620009 | http://www.w3.org/2000/01/rdf-schema#comment | "We recently developed a cell culture system for hepatitis E virus (HEV) in PLC/PRF/5 cells, using a genotype 3 HEV (JE03-1760F strain). Thirteen generations of consecutive passages of culture supernatant were successfully carried out in PLC/PRF/5 cells, with the highest HEV load reaching 10(8) copies/ml in the culture medium. Based on continuous release of progenies into culture medium, 50% tissue culture infectivity doses were estimated to be 2.0 x 10(3) copies for wild-type JE03-1760F and 1.4 x 10(2) copies for p13 (progeny in the thirteenth passage). Earlier appearance and greater increase in the yield of progenies in the culture supernatant were evident in p13 compared with wild-type. The cell culture-produced variants in primary propagation (p0) and consecutive passages (p5 [fifth passage], p10 [tenth], and p13) differed from the wild-type virus by 1, 9, 18, and 19 nucleotides (nt), respectively, over the entire genome of 7226nt, excluding the poly(A) tail. Three of five non-synonymous mutations in p13 were shared by a variant (fifth passage) in another series of passages of JE03-1760F. These results suggest that adaptation of HEV variants to growth in vitro is associated with a limited number of mutations similar to hepatitis A virus."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.virusres.2008.06.005"xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.virusres.2008.06.005"xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.virusres.2008.06.005"xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Hoshino Y."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Hoshino Y."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Hoshino Y."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Inoue J."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Inoue J."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Inoue J."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Okamoto H."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Okamoto H."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Okamoto H."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Takahashi H."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Takahashi H."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Takahashi H."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Tanaka T."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Tanaka T."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Tanaka T."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Takahashi M."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Takahashi M."xsd:string |
| http://purl.uniprot.org/citations/18620009 | http://purl.uniprot.org/core/author | "Takahashi M."xsd:string |