http://purl.uniprot.org/citations/18631005 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/18631005 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundThe myosin heavy chain (MHC) genes are regulated by triiodothyronine (T3) in a reciprocal and chamber-specific manner. To further our understanding of the potential mechanisms involved, we determined the T3 responsiveness of the MHC genes, alpha and beta, and the beta-MHC antisense (AS) gene in the rat ventricles and atria.MethodsHypothyroid rats were administered a single physiologic (1 microg) or pharmacologic (20 microg) dose of T3, and sequential measurements of beta-MHC hn- and AS RNA and alpha-MHC heterogeneous nuclear RNA from rat ventricular and atrial myocardium were performed with reverse transcription PCR.ResultsWe have demonstrated that T3 treatment increases the myocyte content of an AS beta-MHC RNA in atria and ventricles that includes sequences complementary to both the first 5' and last 3' introns of the beta-MHC sense transcript. In the hypothyroid rat ventricle, beta-MHC sense RNA expression is maximal, while in the euthyroid rat ventricle, beta-MHC AS RNA is maximal. beta-MHC AS expression increased by 52 +/-9.8% at the peak, 24 hours after injection of a physiologic dose of T3 (1 microg/animal), while beta-MHC sense RNA decreased by 41 +/-2.2% at 36 hours, the nadir. In hypothyroid atria, beta-MHC AS RNA was induced by threefold within 6 hours of administration of 1 microg T3, demonstrating that in the atria, beta-MHC AS expression is regulated by T3, while alpha-MHC expression is not.ConclusionsIn the hypothyroid rat heart ventricle, beta-MHC AS RNA expression increases in response to T3 similar to that of alpha-MHC. Simultaneous measures of beta-MHC sense RNA are decreased, suggesting a possible mechanism for AS to regulate sense expression. In atria, while alpha-MHC is not influenced by thyroid state, beta-MHC sense and AS RNA were simultaneously and inversely altered in response to T3. This confirms a close positive relationship between T3 and beta-MHC AS RNA in both the atria and ventricles, while demonstrating for the first time that alpha- and beta-MHC expression is not coupled in the atria."xsd:string |
http://purl.uniprot.org/citations/18631005 | http://purl.org/dc/terms/identifier | "doi:10.1089/thy.2008.0043"xsd:string |
http://purl.uniprot.org/citations/18631005 | http://purl.uniprot.org/core/author | "Klein S."xsd:string |
http://purl.uniprot.org/citations/18631005 | http://purl.uniprot.org/core/author | "Klein I."xsd:string |
http://purl.uniprot.org/citations/18631005 | http://purl.uniprot.org/core/author | "Danzi S."xsd:string |
http://purl.uniprot.org/citations/18631005 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
http://purl.uniprot.org/citations/18631005 | http://purl.uniprot.org/core/name | "Thyroid"xsd:string |
http://purl.uniprot.org/citations/18631005 | http://purl.uniprot.org/core/pages | "761-768"xsd:string |
http://purl.uniprot.org/citations/18631005 | http://purl.uniprot.org/core/title | "Differential regulation of the myosin heavy chain genes alpha and beta in rat atria and ventricles: role of antisense RNA."xsd:string |
http://purl.uniprot.org/citations/18631005 | http://purl.uniprot.org/core/volume | "18"xsd:string |
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