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http://purl.uniprot.org/citations/18633119http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18633119http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18633119http://www.w3.org/2000/01/rdf-schema#comment"cpSRP54 (for chloroplast SIGNAL RECOGNITION PARTICLE54) is involved in cotranslational and posttranslational sorting of thylakoid proteins. The Arabidopsis (Arabidopsis thaliana) cpSRP54 null mutant, ffc1-2, is pale green with delayed development. Western-blot analysis of individual leaves showed that the SRP sorting pathway, but not the SecY/E translocon, was strongly down-regulated with progressive leaf development in both wild-type and ffc1-2 plants. To further understand the impact of cpSRP54 deletion, a quantitative comparison of ffc2-1 was carried out for total leaf proteomes of young seedlings and for chloroplast proteomes of fully developed leaves using stable isotope labeling (isobaric stable isotope labeling and isotope-coded affinity tags) and two-dimensional gels. This showed that cpSRP54 deletion led to a change in light-harvesting complex composition, an increase of PsbS, and a decreased photosystem I/II ratio. Moreover, the cpSRP54 deletion led in young leaves to up-regulation of thylakoid proteases and stromal chaperones, including ClpC. In contrast, the stromal protein homeostasis machinery returned to wild-type levels in mature leaves, consistent with the developmental down-regulation of the SRP pathway. A differential response between young and mature leaves was also found in carbon metabolism, with an up-regulation of the Calvin cycle and the photorespiratory pathway in peroxisomes and mitochondria in young leaves but not in old leaves. The Calvin cycle was down-regulated in mature leaves to adjust to the reduced capacity of the light reaction, while reactive oxygen species defense proteins were up-regulated. The significance of ClpC up-regulation was confirmed through the generation of an ffc2-1 clpc1 double mutant. This mutant was seedling lethal under autotrophic conditions but could be partially rescued under heterotrophic conditions."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.org/dc/terms/identifier"doi:10.1104/pp.108.124545"xsd:string
http://purl.uniprot.org/citations/18633119http://purl.org/dc/terms/identifier"doi:10.1104/pp.108.124545"xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/author"Friso G."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/author"Friso G."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/author"Nilsson R."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/author"Nilsson R."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/author"Rutschow H."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/author"Rutschow H."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/author"Ytterberg A.J."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/author"Ytterberg A.J."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/author"van Wijk K.J."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/author"van Wijk K.J."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/name"Plant Physiol."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/name"Plant Physiol."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/pages"156-175"xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/pages"156-175"xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/title"Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/title"Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis."xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/volume"148"xsd:string
http://purl.uniprot.org/citations/18633119http://purl.uniprot.org/core/volume"148"xsd:string
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