| http://purl.uniprot.org/citations/18635542 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18635542 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18635542 | http://www.w3.org/2000/01/rdf-schema#comment | "Nucleoside diphosphate kinase (NDPK/Nm23), responsible for intracellular di- and triphosphonucleoside homeostasis, plays multiple roles in cellular energetics, signaling, proliferation, differentiation and tumor invasion. The only human NDPK with a mitochondrial targeting sequence is NDPK-D, the NME4 gene product, which is a peripheral protein of mitochondrial membranes. Subfractionation of rat liver and HEK 293 cell mitochondria revealed that NDPK-D is essentially bound to the inner membrane. Surface plasmon resonance analysis of the interaction using recombinant NDPK-D and model liposomes showed that NDPK-D interacts electrostatically with anionic phospholipids, with highest affinity observed for cardiolipin. Mutation of the central arginine (Arg-90) in a surface-exposed basic RRK motif unique to NDPK-D strongly reduced interaction with anionic phospholipids. Due to its symmetrical hexameric structure, NDPK-D was able to cross-link anionic phospholipid-containing liposomes, suggesting that NDPK-D could promote intermembrane contacts. Latency assays with isolated mitochondria and antibody binding to mitoplasts indicated a dual orientation for NDPK-D. In HeLa cells, stable expression of wild type but not of the R90D mutant led to membrane-bound enzyme in vivo. Respiration was significantly stimulated by the NDPK substrate TDP in mitochondria containing wild-type NDPK-D, but not in those expressing the R90D mutant, which is catalytically equally active. This indicates local ADP regeneration in the mitochondrial intermembrane space and a tight functional coupling of NDPK-D with oxidative phosphorylation that depends on its membrane-bound state."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m803132200"xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m803132200"xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Schlattner U."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Schlattner U."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Lacombe M.L."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Lacombe M.L."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Borot C."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Borot C."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Speer O."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Speer O."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Munier A."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Munier A."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Boissan M."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Boissan M."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Mailleau C."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Mailleau C."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Tokarska-Schlattner M."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/author | "Tokarska-Schlattner M."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/18635542 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |