| http://purl.uniprot.org/citations/18644383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18644383 | http://www.w3.org/2000/01/rdf-schema#comment | "In an attempt to understand ribosome-induced GTP hydrolysis on eEF2, we determined a 12.6-A cryo-electron microscopy reconstruction of the eEF2-bound 80S ribosome in the presence of aluminum tetrafluoride and GDP, with aluminum tetrafluoride mimicking the gamma-phosphate during hydrolysis. This is the first visualization of a structure representing a transition-state complex on the ribosome. Tight interactions are observed between the factor's G domain and the large ribosomal subunit, as well as between domain IV and an intersubunit bridge. In contrast, some of the domains of eEF2 implicated in small subunit binding display a large degree of flexibility. Furthermore, we find support for a transition-state model conformation of the switch I region in this complex where the reoriented switch I region interacts with a conserved rRNA region of the 40S subunit formed by loops of the 18S RNA helices 8 and 14. This complex is structurally distinct from the eEF2-bound 80S ribosome complexes previously reported, and analysis of this map sheds light on the GTPase-coupled translocation mechanism."xsd:string |
| http://purl.uniprot.org/citations/18644383 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2008.07.004"xsd:string |
| http://purl.uniprot.org/citations/18644383 | http://purl.uniprot.org/core/author | "Frank J."xsd:string |
| http://purl.uniprot.org/citations/18644383 | http://purl.uniprot.org/core/author | "Nilsson J."xsd:string |
| http://purl.uniprot.org/citations/18644383 | http://purl.uniprot.org/core/author | "Nissen P."xsd:string |
| http://purl.uniprot.org/citations/18644383 | http://purl.uniprot.org/core/author | "Kjeldgaard M."xsd:string |
| http://purl.uniprot.org/citations/18644383 | http://purl.uniprot.org/core/author | "Gursky R."xsd:string |
| http://purl.uniprot.org/citations/18644383 | http://purl.uniprot.org/core/author | "Sengupta J."xsd:string |
| http://purl.uniprot.org/citations/18644383 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18644383 | http://purl.uniprot.org/core/name | "J Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/18644383 | http://purl.uniprot.org/core/pages | "179-187"xsd:string |
| http://purl.uniprot.org/citations/18644383 | http://purl.uniprot.org/core/title | "Visualization of the eEF2-80S ribosome transition-state complex by cryo-electron microscopy."xsd:string |
| http://purl.uniprot.org/citations/18644383 | http://purl.uniprot.org/core/volume | "382"xsd:string |
| http://purl.uniprot.org/citations/18644383 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18644383 |
| http://purl.uniprot.org/citations/18644383 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18644383 |
| http://purl.uniprot.org/uniprot/#_P32324-mappedCitation-18644383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18644383 |
| http://purl.uniprot.org/uniprot/#_P60339-mappedCitation-18644383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18644383 |
| http://purl.uniprot.org/uniprot/P32324 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18644383 |
| http://purl.uniprot.org/uniprot/P60339 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18644383 |