| http://purl.uniprot.org/citations/18647841 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18647841 | http://www.w3.org/2000/01/rdf-schema#comment | "The origin recognition complex (ORC) is conserved in all eukaryotes. The six proteins of the Saccharomyces cerevisiae ORC that form a stable complex bind to origins of DNA replication and recruit prereplicative complex (pre-RC) proteins, one of which is Cdc6. To further understand the function of ORC we recently determined by single-particle reconstruction of electron micrographs a low-resolution, 3D structure of S. cerevisiae ORC and the ORC-Cdc6 complex. In this article, the spatial arrangement of the ORC subunits within the ORC structure is described. In one approach, a maltose binding protein (MBP) was systematically fused to the N or the C termini of the five largest ORC subunits, one subunit at a time, generating 10 MBP-fused ORCs, and the MBP density was localized in the averaged, 2D EM images of the MBP-fused ORC particles. Determining the Orc1-5 structure and comparing it with the native ORC structure localized the Orc6 subunit near Orc2 and Orc3. Finally, subunit-subunit interactions were determined by immunoprecipitation of ORC subunits synthesized in vitro. Based on the derived ORC architecture and existing structures of archaeal Orc1-DNA structures, we propose a model for ORC and suggest how ORC interacts with origin DNA and Cdc6. The studies provide a basis for understanding the overall structure of the pre-RC."xsd:string |
| http://purl.uniprot.org/citations/18647841 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.0803829105"xsd:string |
| http://purl.uniprot.org/citations/18647841 | http://purl.uniprot.org/core/author | "Chen Z."xsd:string |
| http://purl.uniprot.org/citations/18647841 | http://purl.uniprot.org/core/author | "Li H."xsd:string |
| http://purl.uniprot.org/citations/18647841 | http://purl.uniprot.org/core/author | "Tang C."xsd:string |
| http://purl.uniprot.org/citations/18647841 | http://purl.uniprot.org/core/author | "Stillman B."xsd:string |
| http://purl.uniprot.org/citations/18647841 | http://purl.uniprot.org/core/author | "Speck C."xsd:string |
| http://purl.uniprot.org/citations/18647841 | http://purl.uniprot.org/core/author | "Wendel P."xsd:string |
| http://purl.uniprot.org/citations/18647841 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18647841 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/18647841 | http://purl.uniprot.org/core/pages | "10326-10331"xsd:string |
| http://purl.uniprot.org/citations/18647841 | http://purl.uniprot.org/core/title | "The architecture of the DNA replication origin recognition complex in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/18647841 | http://purl.uniprot.org/core/volume | "105"xsd:string |
| http://purl.uniprot.org/citations/18647841 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18647841 |
| http://purl.uniprot.org/citations/18647841 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18647841 |
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| http://purl.uniprot.org/uniprot/#_P54791-mappedCitation-18647841 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18647841 |
| http://purl.uniprot.org/uniprot/P54790 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18647841 |
| http://purl.uniprot.org/uniprot/P38826 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18647841 |
| http://purl.uniprot.org/uniprot/P32833 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18647841 |