| http://purl.uniprot.org/citations/18650425 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18650425 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18650425 | http://www.w3.org/2000/01/rdf-schema#comment | "Hypoxia/reoxygenation stress induces the activation of specific signaling proteins and activator protein 1 (AP-1) to regulate cell cycle regression and apoptosis. In the present study, we report that hypoxia/reoxygenation stress activates AP-1 by increasing c-Jun phosphorylation and DNA binding activity through activation of Polo-like-kinase 3 (Plk3) resulting in apoptosis. The specific effect of hypoxia/reoxygenation stress on Plk3 activation resulting in c-Jun phosphorylation was the opposite of UV irradiation-induced responses that are meanly independent on activation of the stress-induced JNK signaling pathway in human corneal epithelial (HCE) cells. The effect of hypoxia/reoxygenation stress-induced Plk3 activation on increased c-Jun phosphorylation and apoptosis was also mimicked by exposure of cells to CoCl(2). Hypoxia/reoxygenation activated Plk3 in HCE cells to directly phosphorylate c-Jun proteins at phosphorylation sites Ser-63 and Ser-73, and to increase DNA binding activity of c-Jun, detected by EMSA. Further evidence demonstrated that Plk3 and phospho-c-Jun were immunocolocalized in the nuclear compartment of hypoxia/reoxygenation stress-induced cells. Increased Plk3 activity by overexpression of wild-type and dominantly positive Plk3 enhanced the effect of hypoxia/reoxygenation on c-Jun phosphorylation and cell death. In contrast, knocking-down Plk3 mRNA suppressed hypoxia-induced c-Jun phosphorylation. Our results provide a new mechanism indicating that hypoxia/reoxygenation induces Plk3 activation instead of the JNK effect to directly phosphorylate and activate c-Jun, subsequently contributing to apoptosis in HCE cells."xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m801326200"xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m801326200"xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/author | "Gao J."xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/author | "Gao J."xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/author | "Lu L."xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/author | "Lu L."xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/author | "Wang L."xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/author | "Wang L."xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/author | "Dai W."xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/author | "Dai W."xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/pages | "25928-25935"xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/pages | "25928-25935"xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/title | "Activation of Polo-like kinase 3 by hypoxic stresses."xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/title | "Activation of Polo-like kinase 3 by hypoxic stresses."xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/volume | "283"xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://purl.uniprot.org/core/volume | "283"xsd:string |
| http://purl.uniprot.org/citations/18650425 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18650425 |
| http://purl.uniprot.org/citations/18650425 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18650425 |