| http://purl.uniprot.org/citations/18650938 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18650938 | http://www.w3.org/2000/01/rdf-schema#comment | "The homotypic fusion of yeast vacuoles, each with 3Q- and 1R-SNARE, requires SNARE chaperones (Sec17p/Sec18p and HOPS) and regulatory lipids (sterol, diacylglycerol and phosphoinositides). Pairs of liposomes of phosphatidylcholine/phosphatidylserine, bearing three vacuolar Q-SNAREs on one and the R-SNARE on the other, undergo slow lipid mixing, but this is unaffected by HOPS and inhibited by Sec17p/Sec18p. To study these essential fusion components, we reconstituted proteoliposomes of a more physiological composition, bearing vacuolar lipids and all four vacuolar SNAREs. Their fusion requires Sec17p/Sec18p and HOPS, and each regulatory lipid is important for rapid fusion. Although SNAREs can cause both fusion and lysis, fusion of these proteoliposomes with Sec17p/Sec18p and HOPS is not accompanied by lysis. Sec17p/Sec18p, which disassemble SNARE complexes, and HOPS, which promotes and proofreads SNARE assembly, act synergistically to form fusion-competent SNARE complexes, and this synergy requires phosphoinositides. This is the first chemically defined model of the physiological interactions of these conserved fusion catalysts."xsd:string |
| http://purl.uniprot.org/citations/18650938 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2008.139"xsd:string |
| http://purl.uniprot.org/citations/18650938 | http://purl.uniprot.org/core/author | "Xu H."xsd:string |
| http://purl.uniprot.org/citations/18650938 | http://purl.uniprot.org/core/author | "Jun Y."xsd:string |
| http://purl.uniprot.org/citations/18650938 | http://purl.uniprot.org/core/author | "Mima J."xsd:string |
| http://purl.uniprot.org/citations/18650938 | http://purl.uniprot.org/core/author | "Wickner W."xsd:string |
| http://purl.uniprot.org/citations/18650938 | http://purl.uniprot.org/core/author | "Hickey C.M."xsd:string |
| http://purl.uniprot.org/citations/18650938 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18650938 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/18650938 | http://purl.uniprot.org/core/pages | "2031-2042"xsd:string |
| http://purl.uniprot.org/citations/18650938 | http://purl.uniprot.org/core/title | "Reconstituted membrane fusion requires regulatory lipids, SNAREs and synergistic SNARE chaperones."xsd:string |
| http://purl.uniprot.org/citations/18650938 | http://purl.uniprot.org/core/volume | "27"xsd:string |
| http://purl.uniprot.org/citations/18650938 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18650938 |
| http://purl.uniprot.org/citations/18650938 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18650938 |
| http://purl.uniprot.org/uniprot/P18759#attribution-A72E43B97BCDC5C0D41CF9804D63EDB1 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/18650938 |
| http://purl.uniprot.org/uniprot/P32602#attribution-A72E43B97BCDC5C0D41CF9804D63EDB1 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/18650938 |
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| http://purl.uniprot.org/uniprot/#_P32602-mappedCitation-18650938 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18650938 |
| http://purl.uniprot.org/uniprot/P32602 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18650938 |
| http://purl.uniprot.org/uniprot/P18759 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18650938 |