| http://purl.uniprot.org/citations/18658147 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18658147 | http://www.w3.org/2000/01/rdf-schema#comment | "The electrogenic sodium bicarbonate cotransporter NBCe1-A mediates the basolateral absorption of sodium and bicarbonate in the proximal tubule. In this study the oligomeric state and minimal functional unit of NBCe1-A were investigated. Wild-type (wt) NBCe1-A isolated from mouse kidney or heterologously expressed in HEK293 cells was predominantly in a dimeric state as was shown using fluorescence energy transfer, pulldown, immunoprecipitation, cross-linking experiments, and nondenaturing perfluorooctanoate-PAGE. NBCe1-A monomers were found to be covalently linked by S-S bonds. When each of the 15 native cysteine residues were individually removed on a wt-NBCe1-A backbone, dimerization of the cotransporter was not affected. In experiments involving multiple native cysteine residue removal, both Cys(630) and Cys(642) in extracellular loop 3 were shown to mediate S-S bond formation between NBCe1-A monomers. When native NBCe1-A cysteine residues were individually reintroduced into a cysteineless NBCe1-A mutant backbone, the finding that a Cys(992) construct that lacked S-S bonds functioned normally indicated that stable covalent linkage of NBCe1-A monomers was not a necessary requirement for functional activity of the cotransporter. Studies using concatameric constructs of wt-NBCe1-A, whose activity is resistant to methanesulfonate reagents, and an NBCe1-A(T442C) mutant, whose activity is completely inhibited by methanesulfonate reagents, confirmed that NBCe1-A monomers are functional. Our results demonstrate that wt-NBCe1-A is predominantly a homodimer, dependent on S-S bond formation that is composed of functionally active monomers."xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m804006200"xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/author | "Liu W."xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/author | "Newman D."xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/author | "Abuladze N."xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/author | "Kurtz I."xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/author | "Pushkin A."xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/author | "Sassani P."xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/author | "Peti-Peterdi J."xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/author | "Azimov R."xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/author | "Kao L."xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/pages | "26782-26794"xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/title | "Oligomeric structure and minimal functional unit of the electrogenic sodium bicarbonate cotransporter NBCe1-A."xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://purl.uniprot.org/core/volume | "283"xsd:string |
| http://purl.uniprot.org/citations/18658147 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18658147 |
| http://purl.uniprot.org/citations/18658147 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18658147 |
| http://purl.uniprot.org/uniprot/#_A7E1Z5-mappedCitation-18658147 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18658147 |
| http://purl.uniprot.org/uniprot/#_E1AWU4-mappedCitation-18658147 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18658147 |
| http://purl.uniprot.org/uniprot/#_E3VWA1-mappedCitation-18658147 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18658147 |
| http://purl.uniprot.org/uniprot/#_A2VDF7-mappedCitation-18658147 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18658147 |
| http://purl.uniprot.org/uniprot/#_E9Q8N8-mappedCitation-18658147 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18658147 |
| http://purl.uniprot.org/uniprot/#_O88343-mappedCitation-18658147 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18658147 |