| http://purl.uniprot.org/citations/18672896 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18672896 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18672896 | http://www.w3.org/2000/01/rdf-schema#comment | "It is becoming increasingly evident that the cell-cell junction is a major signaling center. Here we show that the Galphaq/11 subunit of heterotrimeric G proteins forms a complex with platelet-endothelial cell adhesion molecule 1 (PECAM-1), a junctional protein that has been shown to be involved in mechanosignaling in endothelial cells. To understand the role of PECAM-1 in this complex, we determined the critical regions of PECAM-1 involved in this interaction. By expressing truncated forms of PECAM-1 in human embryonic kidney (HEK293) cells, we found that the cytoplasmic domain of PECAM-1 is not required for its association with Galphaq/11. Domain swapping of PECAM-1 with intracellular cell adhesion molecule 1 (ICAM-1), a protein that does not form a complex with Galphaq/11, provides evidence that the extracellular domain of PECAM-1 is critical for this interaction. This result also suggests that PECAM-1 does not directly interact with Galphaq/11. Coexpression of bradykinin receptor B2 (BKRB2), a Galphaq/11-coupled receptor, with PECAM-1 enhances formation of the PECAM-1-Galphaq/11 complex, suggesting an interaction between PECAM-1 and BKRB2. Co-immunoprecipitation experiments indicate that these two molecules indeed form a complex when expressed in HEK293 cells. Activation of ERK1/2 by bradykinin in HUVEC is enhanced when PECAM-1 expression is inhibited by transfection of small interference RNA against PECAM-1. Taken together, our results provide evidence of interaction of PECAM-1 with BKRB2 and of its possible role in regulating G protein-coupled receptor (GPCR) and G protein functions."xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi8003846"xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi8003846"xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/author | "Yeh J.C."xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/author | "Yeh J.C."xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/author | "Frangos J.A."xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/author | "Frangos J.A."xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/author | "Otte L.A."xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/author | "Otte L.A."xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/pages | "9029-9039"xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/pages | "9029-9039"xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/title | "Regulation of G protein-coupled receptor activities by the platelet-endothelial cell adhesion molecule, PECAM-1."xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/title | "Regulation of G protein-coupled receptor activities by the platelet-endothelial cell adhesion molecule, PECAM-1."xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/volume | "47"xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://purl.uniprot.org/core/volume | "47"xsd:string |
| http://purl.uniprot.org/citations/18672896 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18672896 |
| http://purl.uniprot.org/citations/18672896 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18672896 |
| http://purl.uniprot.org/citations/18672896 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18672896 |
| http://purl.uniprot.org/citations/18672896 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18672896 |