| http://purl.uniprot.org/citations/18679634 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18679634 | http://www.w3.org/2000/01/rdf-schema#comment | "RNA interference mediated by small interfering RNAs (siRNAs) is a powerful tool for evaluating gene function in vivo. In particular it should be able to provide tissue-specific and developmental stage-specific knockdown of target genes in physiological contexts. However, there are few demonstrations of its use on neuronal specific genes in vivo. We recently developed a cationic lipid-based approach to study gene function in a neuronal context. In particular, we applied it to study how the novel partner for TRbeta1, hepatitis virus B X-associated protein 2 (XAP2), a protein first identified as a co-chaperone protein, affects T3-transcriptional repression of the hypothalamic gene, TRH. The cationic lipid-based technique used, JetSI/DOPE, was previously shown to efficiently knockdown reporter gene mRNA in vivo. Using JetSI/DOPE to vectorize siRNA against XAP2 mRNA, we show that XAP2 is needed specifically for TRbeta1-mediated (but not TRbeta2) activation of hypothalamic TRH transcription. Thus, this cationic lipid-based siRNA strategy can effectively be used to reveal fine, tissue-specific and isoform-specific effects on neuronal gene transcription in vivo."xsd:string |
| http://purl.uniprot.org/citations/18679634 | http://purl.org/dc/terms/identifier | "doi:10.1007/978-1-59745-237-3_21"xsd:string |
| http://purl.uniprot.org/citations/18679634 | http://purl.uniprot.org/core/author | "Demeneix B.A."xsd:string |
| http://purl.uniprot.org/citations/18679634 | http://purl.uniprot.org/core/author | "Decherf S."xsd:string |
| http://purl.uniprot.org/citations/18679634 | http://purl.uniprot.org/core/author | "Hassani Z."xsd:string |
| http://purl.uniprot.org/citations/18679634 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18679634 | http://purl.uniprot.org/core/name | "Methods Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/18679634 | http://purl.uniprot.org/core/pages | "355-366"xsd:string |
| http://purl.uniprot.org/citations/18679634 | http://purl.uniprot.org/core/title | "In vivo siRNA delivery to the mouse hypothalamus shows a role of the co-chaperone XAP2 in regulating TRH transcription."xsd:string |
| http://purl.uniprot.org/citations/18679634 | http://purl.uniprot.org/core/volume | "433"xsd:string |
| http://purl.uniprot.org/citations/18679634 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18679634 |
| http://purl.uniprot.org/citations/18679634 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18679634 |
| http://purl.uniprot.org/uniprot/#_O08915-mappedCitation-18679634 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18679634 |
| http://purl.uniprot.org/uniprot/O08915 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18679634 |