| http://purl.uniprot.org/citations/18684992 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18684992 | http://www.w3.org/2000/01/rdf-schema#comment | "K-homology (KH) splicing regulator protein (KSRP) is a multi-domain RNA-binding protein that regulates different steps of mRNA metabolism, from mRNA splicing to mRNA decay, interacting with a broad range of RNA sequences. To understand how KSRP recognizes its different RNA targets it is necessary to define the general rules of KSRP-RNA interaction. We describe here a complete scaffold-independent analysis of the RNA-binding potential of the four KH domains of KSRP. The analysis shows that KH3 binds to the RNA with a significantly higher affinity than the other domains and recognizes specifically a G-rich target. It also demonstrates that the other KH domains of KSRP display different sequence preferences explaining the broad range of targets recognized by the protein. Further, KSRP shows a strong negative selectivity for sequences containing several adjacent Cytosines limiting the target choice of KSRP within single-stranded RNA regions. The in-depth analysis of the RNA-binding potential of the KH domains of KSRP provides us with an understanding of the role of low sequence specificity domains in RNA recognition by multi-domain RNA-binding proteins."xsd:string |
| http://purl.uniprot.org/citations/18684992 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkn509"xsd:string |
| http://purl.uniprot.org/citations/18684992 | http://purl.uniprot.org/core/author | "Ramos A."xsd:string |
| http://purl.uniprot.org/citations/18684992 | http://purl.uniprot.org/core/author | "Garcia-Mayoral M.F."xsd:string |
| http://purl.uniprot.org/citations/18684992 | http://purl.uniprot.org/core/author | "Hollingworth D."xsd:string |
| http://purl.uniprot.org/citations/18684992 | http://purl.uniprot.org/core/author | "Diaz-Moreno I."xsd:string |
| http://purl.uniprot.org/citations/18684992 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18684992 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/18684992 | http://purl.uniprot.org/core/pages | "5290-5296"xsd:string |
| http://purl.uniprot.org/citations/18684992 | http://purl.uniprot.org/core/title | "The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets."xsd:string |
| http://purl.uniprot.org/citations/18684992 | http://purl.uniprot.org/core/volume | "36"xsd:string |
| http://purl.uniprot.org/citations/18684992 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18684992 |
| http://purl.uniprot.org/citations/18684992 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18684992 |
| http://purl.uniprot.org/uniprot/#_Q92945-mappedCitation-18684992 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18684992 |
| http://purl.uniprot.org/uniprot/Q92945 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18684992 |