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http://purl.uniprot.org/citations/18687212http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18687212http://www.w3.org/2000/01/rdf-schema#comment"

Aim

To investigate the change of immunoactivity and surface marker of dendritic cells (DCs) derived from bone marrow and induced by rat 4-1BBL.

Methods

Plasmid pIRES2-EGFP-4-1BBL was constructed and tansfected into HepG2 cells by lipofectin-mediated method, and positive cells were screened by G418. The expression of 4-1BBL in cells were detected by RT-PCR, and the expression of EGFP was detected by fluorescence microscopy. Various HepG2 cells were co-cultured with DC for 2 days, and then the expression of MHC-II and CD80 on DC were detected by FCM, and IL-6 and IL-12 levels secreted by DCs were tested by ELISA.

Results

The recombinant pIRES2-EGFP-4-1BBL vector was successfully constructed and was steadily expressed in HepG2 cells transfected by pIRES2-EGFP-4-1BBL. Compared with HepG2 cells transfected by pIRES2-EGFP plasmid , recombinant HepG2 cells induced high expression of MHC-II and CD80 and secreted more IL-6 and IL-12 on DCs(P<0.05).

Conclusion

The recombinant rat 4-1BBL could promote the maturity of DCs derived from bone marrow."xsd:string
http://purl.uniprot.org/citations/18687212http://purl.uniprot.org/core/author"Chen W.C."xsd:string
http://purl.uniprot.org/citations/18687212http://purl.uniprot.org/core/author"Zhang X.G."xsd:string
http://purl.uniprot.org/citations/18687212http://purl.uniprot.org/core/author"Zhang G.B."xsd:string
http://purl.uniprot.org/citations/18687212http://purl.uniprot.org/core/author"Tan J.X."xsd:string
http://purl.uniprot.org/citations/18687212http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18687212http://purl.uniprot.org/core/name"Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi"xsd:string
http://purl.uniprot.org/citations/18687212http://purl.uniprot.org/core/pages"764-766"xsd:string
http://purl.uniprot.org/citations/18687212http://purl.uniprot.org/core/title"[The change of immunoactivity of dendritic cells induced by recombinant rat 4-1BBL]."xsd:string
http://purl.uniprot.org/citations/18687212http://purl.uniprot.org/core/volume"24"xsd:string
http://purl.uniprot.org/citations/18687212http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18687212
http://purl.uniprot.org/citations/18687212http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/18687212
http://purl.uniprot.org/uniprot/A0A0U5J403#attribution-1E733640A0D9C18D4A858B2D6829D3EEhttp://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/18687212
http://purl.uniprot.org/uniprot/Q80WE6#attribution-1E733640A0D9C18D4A858B2D6829D3EEhttp://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/18687212
http://purl.uniprot.org/uniprot/#_Q80WE6-mappedCitation-18687212http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18687212
http://purl.uniprot.org/uniprot/Q80WE6http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/18687212