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http://purl.uniprot.org/citations/18698677http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Aim

To investigate the effects of lentivirus vector mediated short hairpin RNA interference targeting methionine adenosyltransferase 2beta gene (LV-shMAT2B) on hepatocelluar carcinoma (HCC) cells.

Methods

We constructed four plasmids of RNA interference targeting the MAT2B gene. After LV-shMAT2B was transfected with L-02 cells and two kinds of HCC cells, cell viability and proliferation were measured with MTT and [3H]thymidine assays respectively. Flow cytometry was used to assess cell apoptosis. The level of S-adenosyl methionine (SAMe) in HepG2 cells was evaluated. The expressions of cyclin D1, cyclin D2, bcl-x(L) and bcl-x(S) were detected with western blot.

Results

We constructed LV-shMAT2B successfully. LV-shMAT2B was safe for human normal liver cells. LV-shMAT2B caused dramatic reduction in proliferation compared with controls in HCC cells Bel-7402 (P = 0.054) and HepG2 (P = 0.031). Flow cytometry analysis showed that cell apoptosis caused by LV-shMAT2B was greater in HCC cells Bel-7402 and HepG2 than in control induced by scrambled siRNA (P = 0.047), but apoptosis rates in L-02 induced by LV-shMAT2B and scrambled siRNA respectively had no significant difference. Moreover, LV-shMAT2B significantly suppressed expression of MAT2B leading to growth-inhibition effect on HCC cells by down-regulating cyclin D1. Apoptosis induced by LV-shMAT2B was involved in down-regulating bcl-x(L) and up-regulating bcl-x(S).

Conclusion

LV-shMAT2B can induce cell apoptosis and growth-inhibition in HCC cells. MAT2B may be a therapy target in HCC in the future."xsd:string
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http://purl.uniprot.org/citations/18698677http://purl.uniprot.org/core/author"Liu Q.Y."xsd:string
http://purl.uniprot.org/citations/18698677http://purl.uniprot.org/core/author"Sun Q."xsd:string
http://purl.uniprot.org/citations/18698677http://purl.uniprot.org/core/author"Qian Q."xsd:string
http://purl.uniprot.org/citations/18698677http://purl.uniprot.org/core/author"Wang Q."xsd:string
http://purl.uniprot.org/citations/18698677http://purl.uniprot.org/core/author"Liu Z.S."xsd:string
http://purl.uniprot.org/citations/18698677http://purl.uniprot.org/core/author"Pan D.Y."xsd:string
http://purl.uniprot.org/citations/18698677http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18698677http://purl.uniprot.org/core/name"World J Gastroenterol"xsd:string
http://purl.uniprot.org/citations/18698677http://purl.uniprot.org/core/pages"4633-4642"xsd:string
http://purl.uniprot.org/citations/18698677http://purl.uniprot.org/core/title"Lentivirus mediated shRNA interference targeting MAT2B induces growth-inhibition and apoptosis in hepatocelluar carcinoma."xsd:string
http://purl.uniprot.org/citations/18698677http://purl.uniprot.org/core/volume"14"xsd:string
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