Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/18700867http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18700867http://www.w3.org/2000/01/rdf-schema#comment"

Objectives

A number of stimuli induce cardiac hypertrophy and may lead to cardiomyopathy and heart failure. It is believed that cardiomyocytes withdraw from the cell cycle shortly after birth and become terminally differentiated. However, cell cycle regulatory proteins take part in the development of hypertrophy, and it is important to elucidate the mechanisms of how these proteins are involved in the hypertrophic response in cardiomyocytes.

Materials and methods, and results

In the present study, by immunohistochemistry with a phosphorylation-specific antibody, we found that cyclin D-cdk4/6-phosphorylated retinoblastoma protein (pRb) during hypertrophy and expression of an unphosphorylatable pRb mutant impaired hypertrophic growth in cardiomyocytes. Transcription factor E2F was activated by hypertrophic elicitors but activation was impaired by pharmacological inhibition of cyclin D-cdk4/6. Inhibition of cyclin E-cdk2 complex only partly impaired E2F activity and did not prevent hypertrophic growth, but diminished endoreplication during hypertrophy.

Conclusions

These results indicate that cyclin D-cdk4/6-dependent phosphorylation of pRb and activation of E2F is necessary for hypertrophic growth in cardiomyocytes, whereas cyclin E-cdk2 kinase is not necessary for hypertrophy but regulates endoreplication in these cells. The data support the notion that hypertrophic growth of cardiomyocytes involves a partial progression through the G1 phase of the cell cycle"xsd:string
http://purl.uniprot.org/citations/18700867http://purl.org/dc/terms/identifier"doi:10.1111/j.1365-2184.2008.00549.x"xsd:string
http://purl.uniprot.org/citations/18700867http://purl.uniprot.org/core/author"Busk P.K."xsd:string
http://purl.uniprot.org/citations/18700867http://purl.uniprot.org/core/author"Hansen A.H."xsd:string
http://purl.uniprot.org/citations/18700867http://purl.uniprot.org/core/author"Haunso S."xsd:string
http://purl.uniprot.org/citations/18700867http://purl.uniprot.org/core/author"Hinrichsen R."xsd:string
http://purl.uniprot.org/citations/18700867http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18700867http://purl.uniprot.org/core/name"Cell Prolif"xsd:string
http://purl.uniprot.org/citations/18700867http://purl.uniprot.org/core/pages"813-829"xsd:string
http://purl.uniprot.org/citations/18700867http://purl.uniprot.org/core/title"Phosphorylation of pRb by cyclin D kinase is necessary for development of cardiac hypertrophy."xsd:string
http://purl.uniprot.org/citations/18700867http://purl.uniprot.org/core/volume"41"xsd:string
http://purl.uniprot.org/citations/18700867http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18700867
http://purl.uniprot.org/citations/18700867http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/18700867
http://purl.uniprot.org/uniprot/#_A0A8L2QF92-mappedCitation-18700867http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18700867
http://purl.uniprot.org/uniprot/#_A6HQS0-mappedCitation-18700867http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18700867
http://purl.uniprot.org/uniprot/#_A6HTQ3-mappedCitation-18700867http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18700867
http://purl.uniprot.org/uniprot/#_A6HYI6-mappedCitation-18700867http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18700867
http://purl.uniprot.org/uniprot/#_A6HYI8-mappedCitation-18700867http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18700867
http://purl.uniprot.org/uniprot/#_A6HYI9-mappedCitation-18700867http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18700867
http://purl.uniprot.org/uniprot/#_Q71VC8-mappedCitation-18700867http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18700867
http://purl.uniprot.org/uniprot/#_P33568-mappedCitation-18700867http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18700867
http://purl.uniprot.org/uniprot/#_P39948-mappedCitation-18700867http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18700867
http://purl.uniprot.org/uniprot/#_P35426-mappedCitation-18700867http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18700867
http://purl.uniprot.org/uniprot/#_Q1JUA4-mappedCitation-18700867http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18700867
▶