| http://purl.uniprot.org/citations/18710396 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18710396 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18710396 | http://www.w3.org/2000/01/rdf-schema#comment | "Cellobiose 2-epimerase (CE; EC 5.1.3.11) is known to catalyze the reversible epimerization of cellobiose to 4-O-beta-D-glucopyranosyl-D-mannose in Ruminococcus albus cells. Here, we report a CE in a ruminal strain of Eubacterium cellulosolvens for the first time. The nucleotide sequence of the CE had an ORF of 1218 bp (405 amino acids; 46 963.3 Da). The CE from E. cellulosolvens showed 44-54% identity to N-acyl-D-glucosamine 2-epimerase-like hypothetical proteins in the genomes of Coprococcus eutactus, Faecalibacterium prausnitzii, Clostridium phytofermentans, Caldicellulosiruptor saccharolyticus, and Eubacterium siraeum. Surprisingly, it exhibited only 46% identity to a CE from R. albus. The recombinant enzyme expressed in Escherichia coli was purified by two-step chromatography. The purified enzyme had a molecular mass of 46.7 kDa and exhibited optimal activity at around 35 degrees C and pH 7.0-8.5. In addition to cello-oligosaccharides, it converted lactose to epilactose (4-O-beta-D-galactopyranosyl-D-mannose)."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1574-6968.2008.01281.x"xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1574-6968.2008.01281.x"xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Hamada S."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Hamada S."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Ito S."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Ito S."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Kobayashi Y."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Kobayashi Y."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Matsui H."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Matsui H."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Watanabe J."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Watanabe J."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Taguchi H."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Taguchi H."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Senoura T."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Senoura T."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Wasaki J."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/author | "Wasaki J."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/name | "FEMS Microbiol. Lett."xsd:string |
| http://purl.uniprot.org/citations/18710396 | http://purl.uniprot.org/core/name | "FEMS Microbiol. Lett."xsd:string |