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http://purl.uniprot.org/citations/18718025http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18718025http://www.w3.org/2000/01/rdf-schema#comment"

Introduction

Poly (ADP-ribose) polymerase (PARP) participates in inflammation by cellular necrosis and the nuclear factor-kappa-B (NF-kappaB)-dependent transcription. The purpose of this study was to examine the roles of PARP in ventilator-induced lung injury (VILI) in normal mice lung.

Methods

Male C57BL/6 mice were divided into four groups: sham tracheostomized (sham), lung-protective ventilation (LPV), VILI, and VILI with PARP inhibitor PJ34 pretreatment (PJ34+VILI) groups. Mechanical ventilation (MV) settings were peak inspiratory pressure (PIP) 15 cm H2O + positive end-expiratory pressure (PEEP) 3 cm H2O + 90 breaths per minute for the LPV group and PIP 40 cm H2O + PEEP 0 cm H2O + 90 breaths per minute for the VILI and PJ34+VILI groups. After 2 hours of MV, acute lung injury (ALI) score, wet-to-dry (W/D) weight ratio, PARP activity, and dynamic compliance (CD) were recorded. Tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), myeloperoxidase (MPO) activity, and nitrite/nitrate (NOX) in the bronchoalveolar lavage fluid and NF-kappaB DNA-binding activity in tissue homogenates were measured.

Results

The VILI group showed higher ALI score, W/D weight ratio, MPO activity, NOX, and concentrations of TNF-alpha and IL-6 along with lower CD than the sham and LPV groups (P < 0.05). In the PJ34+VILI group, PJ34 pretreatment improved all histopathologic ALI, inflammatory profiles, and pulmonary dynamics (P < 0.05). NF-kappaB activity was increased in the VILI group as compared with the sham and LPV groups (P < 0.05) and was decreased in the PJ34+VILI group as compared with the VILI group (P = 0.009). Changes in all parameters were closely correlated with the PARP activity (P < 0.05).

Conclusion

Overactivation of PARP plays an important role in the inflammatory and transcriptional pathogenesis of VILI, and PARP inhibition has potentially beneficial effects on the prevention and treatment of VILI."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.org/dc/terms/identifier"doi:10.1186/cc6995"xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"Kim J.H."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"Lee S.Y."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"Kang K.H."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"Jung K.H."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"Kim H.Y."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"Lee S.Y.'"xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"Shin C."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"Yoo S.H."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"Kang E.H."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"Yoon D.W."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"In K.H."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"Shim J.J."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"Suh I.B."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/author"Suk M.H."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/date"2008"xsd:gYear
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/name"Crit Care"xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/pages"R108"xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/title"Inflammatory and transcriptional roles of poly (ADP-ribose) polymerase in ventilator-induced lung injury."xsd:string
http://purl.uniprot.org/citations/18718025http://purl.uniprot.org/core/volume"12"xsd:string
http://purl.uniprot.org/citations/18718025http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/18718025
http://purl.uniprot.org/citations/18718025http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/18718025
http://purl.uniprot.org/uniprot/#_P11103-mappedCitation-18718025http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/18718025
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