| http://purl.uniprot.org/citations/18718449 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18718449 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18718449 | http://www.w3.org/2000/01/rdf-schema#comment | "Itch, an E3 protein ubiquitin ligase (E3), which belongs to the homologous to E6-AP carboxy terminus (HECT)-type subfamily, catalyzes its own ubiquitylation. The precise nature of Itch-mediated self-modification and its biological outcome are not completely understood. Here, we show that Itch auto-ubiquitylation is an intermolecular reaction generating Lys63-linkages, rather than the Lys48-linked polyubiquitin chains that target proteins for proteasomal degradation. As a result, Itch is a relatively high stable protein, whose levels are not significantly affected by treatment by either proteasome or lysosome inhibitors. Furthermore, we demonstrate that the decay rate of a catalytic inactive Itch mutant, which is devoided of self-ubiquitylating activity, is barely indistinguishable from the one of the wild-type protein. These data definitely establish a nondegradative role for Lys63-linked Itch self-ubiquitylation."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bcp.2008.07.028"xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bcp.2008.07.028"xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/author | "Rossi M."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/author | "Rossi M."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/author | "Melino G."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/author | "Melino G."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/author | "Peschiaroli A."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/author | "Peschiaroli A."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/author | "Malatesta M."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/author | "Malatesta M."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/author | "Bernassola F."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/author | "Bernassola F."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/author | "Scialpi F."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/author | "Scialpi F."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/name | "Biochem. Pharmacol."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/name | "Biochem. Pharmacol."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/pages | "1515-1521"xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/pages | "1515-1521"xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/title | "Itch self-polyubiquitylation occurs through lysine-63 linkages."xsd:string |
| http://purl.uniprot.org/citations/18718449 | http://purl.uniprot.org/core/title | "Itch self-polyubiquitylation occurs through lysine-63 linkages."xsd:string |