| http://purl.uniprot.org/citations/18721860 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18721860 | http://www.w3.org/2000/01/rdf-schema#comment | "Rim is a multi-domain, active zone protein that regulates exocytosis and is implicated in vesicle priming and presynaptic plasticity. We recently demonstrated that synaptic defects associated with loss of Caenorhabditis elegans Rim (termed UNC-10) are accompanied by a reduction in docked vesicles adjacent to the presynaptic density. Since Rim is known to interact with the vesicle-associated GTPase Rab3A, here we asked whether UNC-10-dependent recruitment of synaptic vesicles to the presynaptic density was through an UNC-10/Rab-3 interaction. We first established that C. elegans Rab3 (termed RAB-3) in its GTP but not GDP-bound state interacts with UNC-10. We then demonstrated by EM analysis that rab-3 mutant synapses exhibit the same vesicle-targeting defect as unc-10 mutants. Furthermore, unc-10;rab-3 double mutants phenocopy the targeting defects of the single mutants, suggesting UNC-10 and RAB-3 act in the same pathway to target vesicles at the presynaptic density. Endogenous release of unc-10;rab-3 double mutants was similar to that of unc-10 single mutants, but more severe than rab-3 mutants, suggesting the common targeting defects are reflected by the milder rab-3 release defect. Rim has recently been shown to positively regulate calcium influx through direct interactions with calcium channels. Consistent with this notion we found UNC-10 colocalized with the calcium channel, UNC-2 at C. elegans presynaptic densities and synaptic release in unc-10 and rab-3 mutants exhibit reduced calcium-sensitivity. Together these results suggest that vesicles targeted to the presynaptic density by RAB-3/UNC-10 interactions are ideally positioned for efficient calcium-dependent release."xsd:string |
| http://purl.uniprot.org/citations/18721860 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.neulet.2008.08.026"xsd:string |
| http://purl.uniprot.org/citations/18721860 | http://purl.uniprot.org/core/author | "Nonet M.L."xsd:string |
| http://purl.uniprot.org/citations/18721860 | http://purl.uniprot.org/core/author | "Gracheva E.O."xsd:string |
| http://purl.uniprot.org/citations/18721860 | http://purl.uniprot.org/core/author | "Richmond J.E."xsd:string |
| http://purl.uniprot.org/citations/18721860 | http://purl.uniprot.org/core/author | "Hadwiger G."xsd:string |
| http://purl.uniprot.org/citations/18721860 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18721860 | http://purl.uniprot.org/core/name | "Neurosci Lett"xsd:string |
| http://purl.uniprot.org/citations/18721860 | http://purl.uniprot.org/core/pages | "137-142"xsd:string |
| http://purl.uniprot.org/citations/18721860 | http://purl.uniprot.org/core/title | "Direct interactions between C. elegans RAB-3 and Rim provide a mechanism to target vesicles to the presynaptic density."xsd:string |
| http://purl.uniprot.org/citations/18721860 | http://purl.uniprot.org/core/volume | "444"xsd:string |
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