http://purl.uniprot.org/citations/1872611 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/1872611 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/1872611 | http://www.w3.org/2000/01/rdf-schema#comment | "Lactocin S, a bacteriocin produced by Lactobacillus sake L45, has been purified to homogeneity by ion exchange, hydrophobic interaction and reverse-phase chromatography, and gel filtration. The purification resulted in approximately a 40,000-fold increase in the specific activity of lactocin S and enabled the determination of a major part of the amino acid sequence. Judging from the amino acid composition, lactocin S contained approximately 33 amino acid residues, of which about 50% were the nonpolar amino acids alanine, valine, and leucine. Amino acids were not detected upon direct N-terminal sequencing, indicating that the N-terminal amino acid was blocked. By cyanogen bromide cleavage at an internal methionine, the sequence of the 25 amino acids (including the methionine at the cleavage site) in the C-terminal part of the molecule was determined. The sequence was Met-Glu-Leu-Leu-Pro-Thr-Ala-Ala-Val-Leu-Tyr-Xaa-Asp-Val-Ala-Gly-Xaa-Phe-Lys-Tyr-Xaa-Ala-Lys-His-His, where Xaa represents unidentified residues. It is likely that the unidentified residues are modified forms of cysteine or amino acids associated with cysteine, since two cysteic acids per lactocin S molecule were found upon performic acid oxidation of lactocin S. The sequence was unique when compared to the SWISS-PROT data bank."xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.57.6.1829-1834.1991"xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.57.6.1829-1834.1991"xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/author | "Sletten K."xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/author | "Sletten K."xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/author | "Nes I.F."xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/author | "Nes I.F."xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/author | "Mortvedt C.I."xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/author | "Mortvedt C.I."xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/author | "Nissen-Meyer J."xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/author | "Nissen-Meyer J."xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/pages | "1829-1834"xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/pages | "1829-1834"xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/title | "Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45."xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/title | "Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45."xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/volume | "57"xsd:string |
http://purl.uniprot.org/citations/1872611 | http://purl.uniprot.org/core/volume | "57"xsd:string |
http://purl.uniprot.org/citations/1872611 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1872611 |
http://purl.uniprot.org/citations/1872611 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1872611 |