| http://purl.uniprot.org/citations/18762716 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18762716 | http://www.w3.org/2000/01/rdf-schema#comment | "The purpose of this study was to elucidate the mechanism of methyltetrahydrofolate (MTF) transport at the inner blood-retinal barrier (inner BRB). The characteristics and function of MTF transport at the inner BRB were examined using a conditionally immortalized rat retinal capillary endothelial cell line (TR-iBRB2) as an in vitro model of the inner BRB. The [3H]MTF uptake by TR-iBRB2 cells increased with lowering extracellular pH and was Na+- and Cl--independent. The [3H]MTF uptake was concentration-dependent with a K(m) of 5.1 microM. This process was inhibited by reduced folate carrier 1 (RFC1) substrates, such as methotrexate and formyltetrahydrofolate, in a concentration-dependent manner with an IC50 of 8.7 and 2.8 microM, respectively, suggesting that RFC1 mediates MTF uptake in TR-iBRB2 cells. Although both RFC1 and proton-coupled folate transporter (PCFT) mRNA, which are pH-sensitive folate transporters, are expressed in TR-iBRB2 cells and isolated rat retinal vascular endothelial cells, the expression level of RFC1 mRNA was 83- and 49-fold greater than that of PCFT, respectively. Taken together, the above findings are consistent with the involvement of RFC1 in the inner BRB transport of MTF."xsd:string |
| http://purl.uniprot.org/citations/18762716 | http://purl.org/dc/terms/identifier | "doi:10.2133/dmpk.23.285"xsd:string |
| http://purl.uniprot.org/citations/18762716 | http://purl.uniprot.org/core/author | "Fujita K."xsd:string |
| http://purl.uniprot.org/citations/18762716 | http://purl.uniprot.org/core/author | "Hosoya K."xsd:string |
| http://purl.uniprot.org/citations/18762716 | http://purl.uniprot.org/core/author | "Tachikawa M."xsd:string |
| http://purl.uniprot.org/citations/18762716 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18762716 | http://purl.uniprot.org/core/name | "Drug Metab Pharmacokinet"xsd:string |
| http://purl.uniprot.org/citations/18762716 | http://purl.uniprot.org/core/pages | "285-292"xsd:string |
| http://purl.uniprot.org/citations/18762716 | http://purl.uniprot.org/core/title | "Involvement of reduced folate carrier 1 in the inner blood-retinal barrier transport of methyltetrahydrofolate."xsd:string |
| http://purl.uniprot.org/citations/18762716 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/18762716 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18762716 |
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| http://purl.uniprot.org/uniprot/Q62866 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18762716 |
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