| http://purl.uniprot.org/citations/18765284 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18765284 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18765284 | http://www.w3.org/2000/01/rdf-schema#comment | "A wheat germ cell-free extract was used to perform in vitro translation of human stearoyl-CoA desaturase in the presence of unilamelar liposomes, and near complete transfer of the expressed integral membrane protein into the liposome was observed. Moreover, co-translation of the desaturase along with human cytochrome b(5) led to transfer of both membrane proteins into the liposomes. A simple, single step purification via centrifugation in a density gradient yielded proteoliposomes with the desaturase in high purity as judged by capillary electrophoresis. After in vitro reconstitution of the non-heme iron and heme active sites, the function of the reconstituted enzyme complex was demonstrated by conversion of stearoyl-CoA to oleoyl-CoA. This simple translation approach obviates the use of detergents or other lipids to stabilize and isolate a catalytically active integral membrane enzyme. The applicability of cell-free translation to the assembly and purification of other integral membrane protein complexes is discussed."xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.pep.2008.08.002"xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.pep.2008.08.002"xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/author | "Fox B.G."xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/author | "Fox B.G."xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/author | "Goren M.A."xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/author | "Goren M.A."xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/name | "Protein Expr. Purif."xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/name | "Protein Expr. Purif."xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/pages | "171-178"xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/pages | "171-178"xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/title | "Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex."xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/title | "Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex."xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/volume | "62"xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://purl.uniprot.org/core/volume | "62"xsd:string |
| http://purl.uniprot.org/citations/18765284 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18765284 |
| http://purl.uniprot.org/citations/18765284 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18765284 |
| http://purl.uniprot.org/citations/18765284 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18765284 |
| http://purl.uniprot.org/citations/18765284 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18765284 |
| http://purl.uniprot.org/uniprot/O00767 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/18765284 |
| http://purl.uniprot.org/uniprot/O00767#attribution-389113A6FD8FDB5689C347C9517EA1C6 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/18765284 |