DESCRIBE <http://purl.uniprot.org/citations/18772588>

Subject	Predicate	Object
http://purl.uniprot.org/citations/18772588	http://www.w3.org/1999/02/22-rdf-syntax-ns#type	http://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/18772588	http://www.w3.org/2000/01/rdf-schema#comment	" Background Extensive trials have indicated that cancer cells with high glycolytic activity exhibit decreased sensitivity to anticancer agents. Moreover, recent research has proven that specific inhibitors of hexokinase (HK) II, a key glycolytic enzyme, may enhance the activity of anticancer drugs. The aim of this study was to investigate the effect and mechanisms of HK II on chemosensitivity of a colon cancer cell line (LoVo) to 5-fluorouracil (5-FU). Methods HK II gene expression was downregulated by RNA interference in the colon cancer cell line LoVo, which was detected by Western blot analysis. Then the IC(50) value of 5-FU was determined in LoVo cells via MTT assay. In addition, cell apoptosis and mitochondrial membrane potential (MMP) were assessed by flow cytometry and caspase-3 activity by its substrate color reaction. Results In LoVo cells, HK II downregulation resulted in a decreased IC(50) value of 5-FU and increased apoptosis. Furthermore, HK II downregulation resulted in a decreased MPP and activation of caspase-3. Conclusion Our findings suggest that targeting HK II may be beneficial for patients with colon cancer treated with 5-FU."xsd:string
http://purl.uniprot.org/citations/18772588	http://purl.org/dc/terms/identifier	"doi:10.1159/000153655"xsd:string
http://purl.uniprot.org/citations/18772588	http://purl.uniprot.org/core/author	"Zhou Q."xsd:string
http://purl.uniprot.org/citations/18772588	http://purl.uniprot.org/core/author	"Zhou J.M."xsd:string
http://purl.uniprot.org/citations/18772588	http://purl.uniprot.org/core/author	"Pan F."xsd:string
http://purl.uniprot.org/citations/18772588	http://purl.uniprot.org/core/author	"Liang H.J."xsd:string
http://purl.uniprot.org/citations/18772588	http://purl.uniprot.org/core/author	"Peng Q.P."xsd:string
http://purl.uniprot.org/citations/18772588	http://purl.uniprot.org/core/author	"Zhong D.P."xsd:string
http://purl.uniprot.org/citations/18772588	http://purl.uniprot.org/core/date	"2008"xsd:gYear
http://purl.uniprot.org/citations/18772588	http://purl.uniprot.org/core/name	"Chemotherapy"xsd:string
http://purl.uniprot.org/citations/18772588	http://purl.uniprot.org/core/pages	"357-363"xsd:string
http://purl.uniprot.org/citations/18772588	http://purl.uniprot.org/core/title	"Downregulation of the hexokinase II gene sensitizes human colon cancer cells to 5-fluorouracil."xsd:string
http://purl.uniprot.org/citations/18772588	http://purl.uniprot.org/core/volume	"54"xsd:string
http://purl.uniprot.org/citations/18772588	http://www.w3.org/2004/02/skos/core#exactMatch	http://purl.uniprot.org/pubmed/18772588
http://purl.uniprot.org/citations/18772588	http://xmlns.com/foaf/0.1/primaryTopicOf	https://pubmed.ncbi.nlm.nih.gov/18772588
http://purl.uniprot.org/uniprot/#_P52789-mappedCitation-18772588	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/18772588
http://purl.uniprot.org/uniprot/#_A8K2U2-mappedCitation-18772588	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/18772588
http://purl.uniprot.org/uniprot/#_Q53QX9-mappedCitation-18772588	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/18772588
http://purl.uniprot.org/uniprot/#_Q53SG7-mappedCitation-18772588	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/18772588
http://purl.uniprot.org/uniprot/#_Q09LL6-mappedCitation-18772588	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/18772588
http://purl.uniprot.org/uniprot/#_Q68E10-mappedCitation-18772588	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/18772588
http://purl.uniprot.org/uniprot/#_Q7Z7Q6-mappedCitation-18772588	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/18772588
http://purl.uniprot.org/uniprot/Q53QX9	http://purl.uniprot.org/core/mappedCitation	http://purl.uniprot.org/citations/18772588
http://purl.uniprot.org/uniprot/Q09LL6	http://purl.uniprot.org/core/mappedCitation	http://purl.uniprot.org/citations/18772588