| http://purl.uniprot.org/citations/18776696 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18776696 | http://www.w3.org/2000/01/rdf-schema#comment | "Human cystathionine beta-synthase (CBS) catalyzes a pyridoxal 5'-phosphate (PLP) dependent beta-replacement reaction to synthesize cystathionine from serine and homocysteine. The enzyme is unique in bearing not only a catalytically important PLP but also heme. In order to study a regulatory process mediated by heme, we performed mutagenesis of Arg-51 and Arg-224, which have hydrogen-bonding interactions with propionate side chains of the prosthetic group. It was found that the arginine mutations decrease CBS activity by approximately 50%. The results indicate that structural changes in the heme vicinity are transmitted to PLP existing 20 A away from heme. A possible explanation of our results is discussed on the basis of CBS structure."xsd:string |
| http://purl.uniprot.org/citations/18776696 | http://purl.org/dc/terms/identifier | "doi:10.1271/bbb.80231"xsd:string |
| http://purl.uniprot.org/citations/18776696 | http://purl.uniprot.org/core/author | "Sada K."xsd:string |
| http://purl.uniprot.org/citations/18776696 | http://purl.uniprot.org/core/author | "Ozaki S."xsd:string |
| http://purl.uniprot.org/citations/18776696 | http://purl.uniprot.org/core/author | "Inada A."xsd:string |
| http://purl.uniprot.org/citations/18776696 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18776696 | http://purl.uniprot.org/core/name | "Biosci Biotechnol Biochem"xsd:string |
| http://purl.uniprot.org/citations/18776696 | http://purl.uniprot.org/core/pages | "2318-2323"xsd:string |
| http://purl.uniprot.org/citations/18776696 | http://purl.uniprot.org/core/title | "Modulation of cystathionine beta-synthase activity by the Arg-51 and Arg-224 mutations."xsd:string |
| http://purl.uniprot.org/citations/18776696 | http://purl.uniprot.org/core/volume | "72"xsd:string |
| http://purl.uniprot.org/citations/18776696 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18776696 |
| http://purl.uniprot.org/citations/18776696 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18776696 |
| http://purl.uniprot.org/uniprot/P35520#attribution-85D7BA7B94DE65530838BA0D8A3BFED6 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/18776696 |