| http://purl.uniprot.org/citations/18778738 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18778738 | http://www.w3.org/2000/01/rdf-schema#comment | "During cotranslational translocation of proteins into the endoplasmic reticulum (ER) translating ribosomes bind to Sec61-complexes. Presently two models exist how these membrane protein complexes might form protein-conducting channels. While electron microscopic data suggest that a ring-like structure consisting of four Sec61-complexes build the channel, the recently solved crystal structure of a homologous bacterial protein complex led to the speculation that the actual tunnel is formed by just one individual Sec61-complex. Using protease protection assays together with quantitative immunoblotting we directly examined the structure of mammalian protein-conducting channels. We found that in native ER-membranes one single Sec61alpha-molecule is preferentially protected by a membrane bound ribosome, both, in the presence and absence of nascent polypeptides. In addition we present evidence that the nascent polypeptide destabilizes the ring-like translocation apparatus formed by four Sec61-complexes. Moreover, we found that after solubilization of ER-membranes a single Sec61-complex is sufficient to protect the nascent polypeptide chain against added proteases. Finally, we could show that this single Sec61-complex allows the movement of the nascent chain, when it has been released from the ribosome by puromycin treatment. Collectively, our data suggest that the active protein-conducting channel in the ER is formed by a single Sec61-complex."xsd:string |
| http://purl.uniprot.org/citations/18778738 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbamcr.2008.08.005"xsd:string |
| http://purl.uniprot.org/citations/18778738 | http://purl.uniprot.org/core/author | "Hartmann E."xsd:string |
| http://purl.uniprot.org/citations/18778738 | http://purl.uniprot.org/core/author | "Kalies K.U."xsd:string |
| http://purl.uniprot.org/citations/18778738 | http://purl.uniprot.org/core/author | "Stokes V."xsd:string |
| http://purl.uniprot.org/citations/18778738 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18778738 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta"xsd:string |
| http://purl.uniprot.org/citations/18778738 | http://purl.uniprot.org/core/pages | "2375-2383"xsd:string |
| http://purl.uniprot.org/citations/18778738 | http://purl.uniprot.org/core/title | "A single Sec61-complex functions as a protein-conducting channel."xsd:string |
| http://purl.uniprot.org/citations/18778738 | http://purl.uniprot.org/core/volume | "1783"xsd:string |
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