| http://purl.uniprot.org/citations/18801331 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18801331 | http://www.w3.org/2000/01/rdf-schema#comment | "CheY is a response regulator in bacterial chemotaxis. Escherichia coli CheY mutants T87I and T87I/Y106W CheY are phosphorylatable on Asp57 but unable to generate clockwise rotation of the flagella. To understand this phenotype in terms of structure, stable analogs of the two CheY-P mutants were synthesized: T87I phosphono-CheY and T87I phosphono-CheY. Dissociation constants for peptides derived from flagellar motor protein FliM and phosphatase CheZ were determined for phosphono-CheY and the two mutants. The peptides bind phosphono-CheY almost as strongly as CheY-P; however, they do not bind T87I phosphono-CheY or T87I/Y106W phosphono-CheY, implying that the mutant proteins cannot bind FliM or CheZ tightly in vivo. The structures of T87I phosphono-CheY and T87I/Y106W phosphono-CheY were solved to resolutions of 1.8 and 2.4A, respectively. The increased bulk of I87 forces the side-chain of Y106 or W106, into a more solvent-accessible conformation, which occludes the peptide-binding site."xsd:string |
| http://purl.uniprot.org/citations/18801331 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.abb.2008.08.019"xsd:string |
| http://purl.uniprot.org/citations/18801331 | http://purl.uniprot.org/core/author | "Santarsiero B.D."xsd:string |
| http://purl.uniprot.org/citations/18801331 | http://purl.uniprot.org/core/author | "Mesecar A."xsd:string |
| http://purl.uniprot.org/citations/18801331 | http://purl.uniprot.org/core/author | "Eggler A.L."xsd:string |
| http://purl.uniprot.org/citations/18801331 | http://purl.uniprot.org/core/author | "Halkides C.J."xsd:string |
| http://purl.uniprot.org/citations/18801331 | http://purl.uniprot.org/core/author | "Casper E.S."xsd:string |
| http://purl.uniprot.org/citations/18801331 | http://purl.uniprot.org/core/author | "Matthew Haas R."xsd:string |
| http://purl.uniprot.org/citations/18801331 | http://purl.uniprot.org/core/author | "McAdams K."xsd:string |
| http://purl.uniprot.org/citations/18801331 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18801331 | http://purl.uniprot.org/core/name | "Arch Biochem Biophys"xsd:string |
| http://purl.uniprot.org/citations/18801331 | http://purl.uniprot.org/core/pages | "105-113"xsd:string |
| http://purl.uniprot.org/citations/18801331 | http://purl.uniprot.org/core/title | "The structures of T87I phosphono-CheY and T87I/Y106W phosphono-CheY help to explain their binding affinities to the FliM and CheZ peptides."xsd:string |
| http://purl.uniprot.org/citations/18801331 | http://purl.uniprot.org/core/volume | "479"xsd:string |
| http://purl.uniprot.org/citations/18801331 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18801331 |
| http://purl.uniprot.org/citations/18801331 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18801331 |
| http://purl.uniprot.org/uniprot/#_P0AE67-mappedCitation-18801331 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18801331 |
| http://purl.uniprot.org/uniprot/P0AE67 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18801331 |