| http://purl.uniprot.org/citations/18801728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18801728 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18801728 | http://www.w3.org/2000/01/rdf-schema#comment | "The bimolecular interaction between corticotropin-releasing factor (CRF), a neuropeptide, and its type 1 receptor (CRFR1), a class B G-protein-coupled receptor (GPCR), is crucial for activation of the hypothalamic-pituitary-adrenal axis in response to stress, and has been a target of intense drug design for the treatment of anxiety, depression, and related disorders. As a class B GPCR, CRFR1 contains an N-terminal extracellular domain (ECD) that provides the primary ligand binding determinants. Here we present three crystal structures of the human CRFR1 ECD, one in a ligand-free form and two in distinct CRF-bound states. The CRFR1 ECD adopts the alpha-beta-betaalpha fold observed for other class B GPCR ECDs, but the N-terminal alpha-helix is significantly shorter and does not contact CRF. CRF adopts a continuous alpha-helix that docks in a hydrophobic surface of the ECD that is distinct from the peptide-binding site of other class B GPCRs, thereby providing a basis for the specificity of ligand recognition between CRFR1 and other class B GPCRs. The binding of CRF is accompanied by clamp-like conformational changes of two loops of the receptor that anchor the CRF C terminus, including the C-terminal amide group. These structural studies provide a molecular framework for understanding peptide binding and specificity by the CRF receptors as well as a template for designing potent and selective CRFR1 antagonists for therapeutic applications."xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m805749200"xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m805749200"xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/author | "Xu H.E."xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/author | "Xu H.E."xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/author | "Suino-Powell K."xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/author | "Suino-Powell K."xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/author | "Parker N.R."xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/author | "Parker N.R."xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/author | "Pioszak A.A."xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/author | "Pioszak A.A."xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/pages | "32900-32912"xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/pages | "32900-32912"xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/title | "Molecular recognition of corticotropin-releasing factor by its G-protein-coupled receptor CRFR1."xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/title | "Molecular recognition of corticotropin-releasing factor by its G-protein-coupled receptor CRFR1."xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/volume | "283"xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://purl.uniprot.org/core/volume | "283"xsd:string |
| http://purl.uniprot.org/citations/18801728 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18801728 |
| http://purl.uniprot.org/citations/18801728 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18801728 |