| http://purl.uniprot.org/citations/18802028 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18802028 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18802028 | http://www.w3.org/2000/01/rdf-schema#comment | "We previously identified an alpha1-AR-ERK (alpha1A-adrenergic receptor-extracellular signal-regulated kinase) survival signaling pathway in adult cardiac myocytes. Here, we investigated localization of alpha1-AR subtypes (alpha1A and alpha1B) and how their localization influences alpha1-AR signaling in cardiac myocytes. Using binding assays on myocyte subcellular fractions or a fluorescent alpha1-AR antagonist, we localized endogenous alpha1-ARs to the nucleus in wild-type adult cardiac myocytes. To clarify alpha1 subtype localization, we reconstituted alpha1 signaling in cultured alpha1A- and alpha1B-AR double knockout cardiac myocytes using alpha1-AR-green fluorescent protein (GFP) fusion proteins. Similar to endogenous alpha1-ARs and alpha1A- and alpha1B-GFP colocalized with LAP2 at the nuclear membrane. alpha1-AR nuclear localization was confirmed in vivo using alpha1-AR-GFP transgenic mice. The alpha1-signaling partners Galphaq and phospholipase Cbeta1 also colocalized with alpha1-ARs only at the nuclear membrane. Furthermore, we observed rapid catecholamine uptake mediated by norepinephrine-uptake-2 and found that alpha1-mediated activation of ERK was not inhibited by a membrane impermeant alpha1-blocker, suggesting alpha1 signaling is initiated at the nucleus. Contrary to prior studies, we did not observe alpha1-AR localization to caveolae, but we found that alpha1-AR signaling initiated at the nucleus led to activated ERK localized to caveolae. In summary, our results show that nuclear alpha1-ARs transduce signals to caveolae at the plasma membrane in cardiac myocytes."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.org/dc/terms/identifier | "doi:10.1161/circresaha.108.176024"xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.org/dc/terms/identifier | "doi:10.1161/circresaha.108.176024"xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "Chen Q."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "Chen Q."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "Huang Y."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "Huang Y."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "Baye N.L."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "Baye N.L."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "Healy C.L."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "Healy C.L."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "Kasinathan S."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "Kasinathan S."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "O'Connell T.D."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "O'Connell T.D."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "Wright C.D."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/author | "Wright C.D."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/name | "Circ. Res."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/name | "Circ. Res."xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/pages | "992-1000"xsd:string |
| http://purl.uniprot.org/citations/18802028 | http://purl.uniprot.org/core/pages | "992-1000"xsd:string |