| http://purl.uniprot.org/citations/18805091 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18805091 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18805091 | http://www.w3.org/2000/01/rdf-schema#comment | "Formation of single-strand DNA (ssDNA) tails at a double-strand break (DSB) is a key step in homologous recombination and DNA-damage signaling. The enzyme(s) producing ssDNA at DSBs in eukaryotes remain unknown. We monitored 5'-strand resection at inducible DSB ends in yeast and identified proteins required for two stages of resection: initiation and long-range 5'-strand resection. We show that the Mre11-Rad50-Xrs2 complex (MRX) initiates 5' degradation, whereas Sgs1 and Dna2 degrade 5' strands exposing long 3' strands. Deletion of SGS1 or DNA2 reduces resection and DSB repair by single-strand annealing between distant repeats while the remaining long-range resection activity depends on the exonuclease Exo1. In exo1Deltasgs1Delta double mutants, the MRX complex together with Sae2 nuclease generate, in a stepwise manner, only few hundred nucleotides of ssDNA at the break, resulting in inefficient gene conversion and G2/M damage checkpoint arrest. These results provide important insights into the early steps of DSB repair in eukaryotes."xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cell.2008.08.037"xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cell.2008.08.037"xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/author | "Zhu Z."xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/author | "Zhu Z."xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/author | "Lee S.E."xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/author | "Lee S.E."xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/author | "Ira G."xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/author | "Ira G."xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/author | "Chung W.H."xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/author | "Chung W.H."xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/author | "Shim E.Y."xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/author | "Shim E.Y."xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/name | "Cell"xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/name | "Cell"xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/pages | "981-994"xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/pages | "981-994"xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/title | "Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double-strand break ends."xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/title | "Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double-strand break ends."xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/volume | "134"xsd:string |
| http://purl.uniprot.org/citations/18805091 | http://purl.uniprot.org/core/volume | "134"xsd:string |