| http://purl.uniprot.org/citations/18806471 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18806471 | http://www.w3.org/2000/01/rdf-schema#comment | "Double-stranded RNA (dsRNA) induces gene silencing in a sequence-specific manner by a process known as RNA interference (RNAi). The RNA-induced silencing complex (RISC) is a multi-subunit ribonucleoprotein complex that plays a key role in RNAi. VIG (Vasa intronic gene) has been identified as a component of Drosophila RISC; however, the role VIG plays in regulating RNAi is poorly understood. Here, we examined the spatial and temporal expression patterns of VIG-1, the C. elegans ortholog of Drosophila VIG, using a vig-1::gfp fusion construct. This construct contains the 908-bp region immediately upstream of vig-1 gene translation initiation site. Analysis by confocal microscopy demonstrated GFP-VIG-1 expression in a number of tissues including the pharynx, body wall muscle, hypodermis, intestine, reproductive system, and nervous system at the larval and adult stages. Furthermore, western blot analysis showed that VIG-1 is present in each developmental stage examined. To investigate regulatory sequences for vig-1 gene expression, we generated constructs containing deletions in the upstream region. It was determined that the GFP expression pattern of a deletion construct (delta-908 to -597) was generally similar to that of the non-deletion construct. In contrast, removal of a larger segment (delta-908 to -191) resulted in the loss of GFP expression in most cell types. Collectively, these results indicate that the 406-bp upstream region (-596 to -191) contains essential regulatory sequences required for VIG-1 expression."xsd:string |
| http://purl.uniprot.org/citations/18806471 | http://purl.org/dc/terms/identifier | "doi:10.1016/s1016-8478(23)14036-2"xsd:string |
| http://purl.uniprot.org/citations/18806471 | http://purl.uniprot.org/core/author | "Park Y.S."xsd:string |
| http://purl.uniprot.org/citations/18806471 | http://purl.uniprot.org/core/author | "Shin K.H."xsd:string |
| http://purl.uniprot.org/citations/18806471 | http://purl.uniprot.org/core/author | "Cho N.J."xsd:string |
| http://purl.uniprot.org/citations/18806471 | http://purl.uniprot.org/core/author | "Choi B."xsd:string |
| http://purl.uniprot.org/citations/18806471 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18806471 | http://purl.uniprot.org/core/name | "Mol Cells"xsd:string |
| http://purl.uniprot.org/citations/18806471 | http://purl.uniprot.org/core/pages | "554-557"xsd:string |
| http://purl.uniprot.org/citations/18806471 | http://purl.uniprot.org/core/title | "Analysis of C. elegans VIG-1 expression."xsd:string |
| http://purl.uniprot.org/citations/18806471 | http://purl.uniprot.org/core/volume | "26"xsd:string |
| http://purl.uniprot.org/citations/18806471 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18806471 |
| http://purl.uniprot.org/citations/18806471 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18806471 |
| http://purl.uniprot.org/uniprot/#_H2KYR1-mappedCitation-18806471 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18806471 |
| http://purl.uniprot.org/uniprot/H2KYR1 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18806471 |