| http://purl.uniprot.org/citations/18834073 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18834073 | http://www.w3.org/2000/01/rdf-schema#comment | "ObjectivesWe sought to document the mucin gene profile in normal human laryngeal epithelium and compare it with that in patients with reflux-attributed laryngeal injury or disease. We also investigated the effect of low pH with or without pepsin on mucin messenger RNA levels in vitro.MethodsLaryngeal biopsy specimens were obtained from 3 patients with clinically diagnosed laryngopharyngeal reflux and from 2 control subjects who had no signs or symptoms of reflux. Signs and symptoms were assessed by the Reflux Finding Score and the Reflux Symptom Index, respectively. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to establish the mucin gene profile. Human hypopharyngeal epithelial cells were exposed to pH 7, 5, 4, and 2 with and without pepsin (0.1 mg/mL) for 20 minutes at 37 degrees C, and expression of selected mucins was analyzed via real-time RT-PCR.ResultsMucin 1-5, 7, 9, 13, 15, 16, and 18-20 transcripts were detected in normal laryngeal epithelium, whereas mucin 6, 8, and 17 transcripts were not. Mucins 2, 3, and 5 were expressed at reduced levels in patients with reflux-attributed laryngeal injury or disease. These mucin genes were up-regulated after exposure to low pH in vitro (p < 0.005). Pepsin inhibited this up-regulation (p <0.001).ConclusionsReflux laryngitis is associated with down-regulation of mucin gene expression."xsd:string |
| http://purl.uniprot.org/citations/18834073 | http://purl.org/dc/terms/identifier | "doi:10.1177/000348940811700911"xsd:string |
| http://purl.uniprot.org/citations/18834073 | http://purl.uniprot.org/core/author | "Blumin J.H."xsd:string |
| http://purl.uniprot.org/citations/18834073 | http://purl.uniprot.org/core/author | "Johnston N."xsd:string |
| http://purl.uniprot.org/citations/18834073 | http://purl.uniprot.org/core/author | "Pajewski N.M."xsd:string |
| http://purl.uniprot.org/citations/18834073 | http://purl.uniprot.org/core/author | "Kerschner J.E."xsd:string |
| http://purl.uniprot.org/citations/18834073 | http://purl.uniprot.org/core/author | "Handler E."xsd:string |
| http://purl.uniprot.org/citations/18834073 | http://purl.uniprot.org/core/author | "Samuels T.L."xsd:string |
| http://purl.uniprot.org/citations/18834073 | http://purl.uniprot.org/core/author | "Syring M.L."xsd:string |
| http://purl.uniprot.org/citations/18834073 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18834073 | http://purl.uniprot.org/core/name | "Ann Otol Rhinol Laryngol"xsd:string |
| http://purl.uniprot.org/citations/18834073 | http://purl.uniprot.org/core/pages | "688-695"xsd:string |
| http://purl.uniprot.org/citations/18834073 | http://purl.uniprot.org/core/title | "Mucin gene expression in human laryngeal epithelia: effect of laryngopharyngeal reflux."xsd:string |
| http://purl.uniprot.org/citations/18834073 | http://purl.uniprot.org/core/volume | "117"xsd:string |
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