| http://purl.uniprot.org/citations/18835357 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/18835357 | http://www.w3.org/2000/01/rdf-schema#comment | "We used post-transcriptional gene silencing (with small interfering RNA) to examine specifically the roles of Type 1 inositol tris-phosphate receptors (InsP(3)R1) and transient receptor potential channel 6 (TRPC6) in Ca(2+) oscillations induced by arginine vasopressin (AVP), a typical G-protein coupled receptor agonist. Ca(2+) oscillations were observed in individual A7r5 cells with confocal imaging of fluo-4 fluorescence, and SR-releasable Ca(2+) was assessed by exposure to cyclopiazonic acid (CPA). In control cells, both AVP (100 nM) and a direct activator of TRPC6 (OAG, l-oleoyl-2-acetyl-glycerol, 100 microM) caused Ca(2+) oscillations in the majority of cells (e.g. AVP: 85%, 0.97+/-0.05/min; OAG: 83%, 1.00+/-0.07/min). Partial knock-down of TRPC6 (to <27% protein expression) was more effective than partial knock-down of InsP(3)R1 (to <30% protein expression) in reducing the fraction of cells that produced Ca(2+) oscillations in response to AVP or OAG (22% and 83% of cells showing oscillations, respectively, in response to AVP; 31% and 72% of cells showing oscillation, respectively, in response to OAG). CPA-induced SR Ca(2+) release was unaffected by siRNA transfection. Inhibition of InsP(3)R with Xestospongin C abolished both AVP and OAG-induced Ca(2+) oscillations. Nifedipine (10 microM) had no effect. The key results, including the effects of partial (as opposed to complete) knock-down of InsP(3)R1 and TRPC6, and the (unexpected) finding of OAG-induced Ca(2+) oscillations, are predicted by a canonical mathematical model of Ca(2+) oscillations in which InsP(3)R1 functions as the SR Ca(2+) release channel and TRPC6 as the receptor-operated Ca(2+) influx channel. These results indicated that TRPC6 functioning as a major type of receptor-operated Ca(2+) channel played a critical role in Ca(2+) oscillations of A7r5 cells' response to AVP or OAG, and partial knock-down of TRPC6 was more effective than partial knock-down of InsP(3)R1 in reducing Ca(2+) oscillations."xsd:string |
| http://purl.uniprot.org/citations/18835357 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.phrs.2008.09.004"xsd:string |
| http://purl.uniprot.org/citations/18835357 | http://purl.uniprot.org/core/author | "Li M."xsd:string |
| http://purl.uniprot.org/citations/18835357 | http://purl.uniprot.org/core/author | "Sun X."xsd:string |
| http://purl.uniprot.org/citations/18835357 | http://purl.uniprot.org/core/author | "Wier W.G."xsd:string |
| http://purl.uniprot.org/citations/18835357 | http://purl.uniprot.org/core/author | "Zacharia J."xsd:string |
| http://purl.uniprot.org/citations/18835357 | http://purl.uniprot.org/core/date | "2008"xsd:gYear |
| http://purl.uniprot.org/citations/18835357 | http://purl.uniprot.org/core/name | "Pharmacol Res"xsd:string |
| http://purl.uniprot.org/citations/18835357 | http://purl.uniprot.org/core/pages | "308-315"xsd:string |
| http://purl.uniprot.org/citations/18835357 | http://purl.uniprot.org/core/title | "Effects of siRNA knock-down of TRPC6 and InsP(3)R1 in vasopressin-induced Ca(2+) oscillations of A7r5 vascular smooth muscle cells."xsd:string |
| http://purl.uniprot.org/citations/18835357 | http://purl.uniprot.org/core/volume | "58"xsd:string |
| http://purl.uniprot.org/citations/18835357 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/18835357 |
| http://purl.uniprot.org/citations/18835357 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/18835357 |
| http://purl.uniprot.org/uniprot/#_A6IBL0-mappedCitation-18835357 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18835357 |
| http://purl.uniprot.org/uniprot/#_C7E1V2-mappedCitation-18835357 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18835357 |
| http://purl.uniprot.org/uniprot/#_P29994-mappedCitation-18835357 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18835357 |
| http://purl.uniprot.org/uniprot/#_Q5BJS8-mappedCitation-18835357 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/18835357 |
| http://purl.uniprot.org/uniprot/A6IBL0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18835357 |
| http://purl.uniprot.org/uniprot/P29994 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18835357 |
| http://purl.uniprot.org/uniprot/C7E1V2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18835357 |
| http://purl.uniprot.org/uniprot/Q5BJS8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/18835357 |